P物质对表皮细胞聚集、分化控制及在皮肤创伤修复中的应用

发布时间：2019-04-02 12:23

【摘要】：背景:表皮干细胞是皮肤及其附属器修复的源泉细胞,研究其分化、迁移的调控机制是实现其完美愈合的关键。目的:探讨P物质在皮肤创伤修复中的应用效果。方法:采用随机对照方法将30只Wistar大鼠分为P物质组、辣椒素组和对照组,每组10只。建立皮肤缺损模型后分别于创面局部注射外源性神经肽P物质、辣椒素、生理盐水,均连续注射5 d。用药不同时间点检测3组大鼠皮肤厚度、皮肤创伤面积、BrdU阳性细胞率。结果与结论:1用药第14天3组大鼠皮肤厚度差异无显著性意义(P0.05);随着用药时间的不断延长,表皮厚度开始变薄;用药第42天,P物质组表皮厚度明显缩小,对照组表皮厚度变化不明显,辣椒素组表皮厚度有所缩小。2皮肤创伤3,5,7 d时P物质组皮肤创伤面积显著小于对照组和辣椒素组(P0.05),辣椒素组和对照组的创伤面积差异无显著性意义(P0.05)。3P物质组在用药第3,5,7,12天时Brd U细胞阳性率显著高于辣椒素组和对照组(P0.05);辣椒素组在第3,5,7,12天时BrdU细胞阳性率显著低于对照组(P0.05)。4结果提示P物质能够促进表皮细胞增殖,加速创面部位愈合。
[Abstract]:Background: epidermal stem cells are the source cells for repair of skin and its adnexes. The key to perfect healing is to study the differentiation and migration mechanism of epidermal stem cells. Objective: to investigate the effect of substance P in skin wound repair. Methods: 30 Wistar rats were randomly divided into substance P group, capsaicin group and control group with 10 rats in each group. After the skin defect model was established, exogenous neuropeptide substance P, capsaicin and saline were injected into the wound respectively for 5 d. The thickness of skin, the area of skin trauma and the rate of BrdU positive cells were measured at different time points. Results & conclusion: (1) there was no significant difference in skin thickness among the three groups on the 14th day (P0.05), and the thickness of the epidermis began to thin with the prolongation of the medication time. On the 42nd day of treatment, the thickness of epidermis of substance P group decreased obviously, but that of control group did not change obviously, and the thickness of epidermis of capsaicin group decreased somewhat. On the 7th day, the skin trauma area of substance P group was significantly smaller than that of control group and capsaicin group (P0.05), but there was no significant difference between capsaicin group and control group (P0.05). On the 12th day, the positive rate of Brd U cells in capsaicin group and control group was significantly higher than that in capsaicin group and control group (P0.05). The positive rate of BrdU cells in capsaicin group was significantly lower than that in control group on the 3rd, 5th, 7th and 12th day (P0.05). 4 the results suggested that substance P could promote the proliferation of epidermal cells and accelerate wound healing.
【作者单位】：郑州大学附属郑州中心医院;
【基金】：河南省中医药科研专项课题(2014ZY02082)~~
【分类号】：R641

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