依达拉奉对脓毒症大鼠心肌损伤的保护作用

发布时间：2019-05-28 12:50

【摘要】：目的观察依达拉奉对脓毒症大鼠心肌损伤的保护作用。方法 78只清洁级SD大鼠随机分为正常组(Norm组)、假手术组(Sham组)、脓毒症组(CLP组)、小剂量依达拉奉治疗组(ED1组)(3 mg/kg,静脉注射)及大剂量依达拉奉治疗组(ED2组)(6 mg/kg,静脉注射)。盲肠结扎穿孔法制作脓毒症模型,ED1组及ED2组给予依达拉奉治疗。分别于术后3、6及12 h检测各组血清c Tn I和心肌·OH-、丙二醛(MDA)、超氧化物歧化酶(SOD)、肿瘤坏死因子(TNF-α)及白细胞介素1β(IL-1β)水平,并观察心肌病理形态改变。结果 Norm组与Sham组各指标差异无统计学意义(P0.05);CLP组与Norm组及Sham组比较,c Tn I、·OH-、MDA、TNF-α及IL-1β水平明显升高,SOD水平明显降低(P0.01);ED1组及ED2组与CLP组比较c Tn I、·OH-、MDA、TNF-α及IL-1β水平明显降低,SOD水平明显升高(P0.01);ED2组与ED1组比较,c Tn I、·OH-、MDA、TNF-α及IL-1β水平明显降低,SOD水平明显升高(P0.01)。CLP组心肌病理学改变包括间质充血水肿,炎症细胞浸润,心肌纤维断裂、坏死;ED1组及ED2组心肌损伤较CLP组明显减轻。结论 1依达拉奉能减轻脓毒症心肌损伤,机制可能与抑制氧化应激及下调炎症因子水平有关;2大剂量依达拉奉(6 mg/kg)对脓毒症心肌损伤的保护作用较小剂量(3 mg/kg)更明显。
[Abstract]:Objective to observe the protective effect of Edaravone on myocardial injury in septic rats. Methods 78 clean SD rats were randomly divided into normal group (Norm group), sham operation group (Sham group), sepsis group (CLP group) and low dose Edaravone treatment group (ED1 group) (3 mg/kg,). Intravenous injection) and high dose Edaravone treatment group (ED2 group) (6 mg/kg, intravenous injection). Sepsis model was established by cecal ligation and perforation. ED1 group and ED2 group were treated with Edaravone. The levels of serum c Tn I and myocardial OH-, malondialdehyde (MDA), (SOD), tumor necrosis factor (TNF- 伪) and IL 1 尾 (IL-1 尾) were measured at 3 h, 6 h and 12 h after operation, respectively. the levels of MDA, SOD, SOD 伪 and IL-1 尾 were measured at 3 h, 6 h and 12 h after operation. The pathological changes of myocardium were observed. Results there was no significant difference in the indexes between Norm group and Sham group (P 0.05). The levels of, c Tn I, OH-,MDA,TNF- 伪 and IL-1 尾 in); CLP group were significantly higher than those in Norm group and Sham group, while the level of SOD was significantly decreased (P 0.01). Compared with CLP group, the levels of c Tn I, OH-,MDA,TNF- 伪 and IL-1 尾 in ED1 group and ED2 group were significantly lower than those in CLP group, while the levels of SOD in CLP group were significantly higher than those in CLP group (P 0.01). Compared with ED1 group, the levels of, c Tn I, OH-,MDA,TNF- 伪 and IL-1 尾 in ED2 group were significantly lower than those in ED1 group, while the level of SOD in). CLP group was significantly higher (P 0.01). The pathological changes of myocardium in). CLP group included interstitial congestion and edema, inflammatory cell infiltration and myocardial fiber breakage. (B) Necrosis; Myocardial injury in ED1 group and ED2 group was significantly less than that in CLP group. Conclusion (1) Edaravone can alleviate myocardial injury in sepsis, which may be related to the inhibition of oxidative stress and the down-regulation of inflammatory factor level. (2) the protective effect of high dose Edaravone (6 mg/kg) on sepsis myocardial injury was more obvious than that of low dose (3 mg/kg).
【作者单位】：兰州大学第二医院重症医学Ⅱ科;中南大学湘雅医院重症医学科;
【分类号】：R459.7

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