HSP90在脓毒症小鼠中的表达及血必净对其表达的影响

发布时间：2019-06-07 14:23

【摘要】：为了研究热休克蛋白90(HSP90)因子在脓毒症小鼠中的表达水平,并研究血必净干预、治疗对HSP90表达的影响,探讨HSP90因子在脓毒症病程中的生物学意义,试验采用尾静脉注射脂多糖(LPS)建立脓毒症小鼠模型,血必净干预组小鼠腹腔注射10 m L/kg剂量血必净,每日2次,而模型组小鼠予以等量生理盐水处理,采用实时定量PCR(Real time PCR)方法检测两组小鼠心脏和肾脏组织中HSP90 mRNA的相对表达量;采用酶联免疫吸附试验(ELISA)检测两组小鼠血清肌酐(Cr)、血尿素氮(BUN)水平及心脏、肾脏组织液中HSP90因子表达水平;采用H.E.染色观察两组小鼠心脏、肾脏组织病理结构。结果表明:小鼠尾静脉注射LPS后,血清Cr和BUN水平出现持续性增高,于18小时达到最高水平,两组小鼠血清BUN、Cr水平最早于注射LPS造模8 h后出现显著差异(P0.05);在脓毒症小鼠心脏组织中,HSP90 mRNA及蛋白水平均出现明显上调,整体呈先上升后下降的趋势,模型组HSP90 mRNA及蛋白总体水平高于血必净干预组(P0.05,P0.01),HSP90 mRNA及蛋白在脓毒症小鼠肾脏组织中的表达水平及变化趋势与其在心脏组织中相似;脓毒症小鼠心、肾组织发生明显炎性反应,可见组织结构异常,而干预组小鼠保持较为完整的心脏、肾脏组织结构,无显著病理学变化。说明脓毒症小鼠心脏、肾脏组织中的HSP90基因和蛋白的表达随脓毒症发生、发展表现不同程度的上升,最早于造模后2小时出现显著增高,表明HSP90因子参与脓毒症发生、发展病程,提示可将HSP90列为脓毒症临床早期检测、诊断指标之一;血必净干预对HSP90因子具有调节作用,能够有效改善脓毒症症状。
[Abstract]:In order to study the expression of heat shock protein 90 (HSP90) factor in sepsis mice, and to study the effect of Xuebijing intervention on the expression of HSP90, and to explore the biological significance of HSP90 factor in the course of sepsis. The model of sepsis mice was established by intravenous injection of lipopolysaccharide (LPS). The mice in Xuebijing intervention group were intraperitoneally injected with 10 m L/kg Xuebijing twice a day, while the mice in model group were treated with the same amount of saline. The relative expression of HSP90 mRNA in heart and kidney of the two groups was detected by real-time quantitative PCR (Real time PCR). Enzyme linked immunosorbent assay (ELISA) was used to detect the level of serum creatinine (Cr), blood urea nitrogen (BUN) and the expression of HSP90 factor in heart and kidney tissue fluid of the two groups. The pathological structure of heart and kidney in the two groups was observed by staining. The results showed that the levels of serum Cr and BUN increased continuously after intravenous injection of LPS in mice, and reached the highest level at 18 hours. The levels of serum BUN,Cr in the two groups were significantly different from those 8 hours after injection of LPS (P 0.05). In the heart tissue of sepsis mice, the levels of HSP90 mRNA and protein were significantly up-regulated and increased at first and then decreased. The overall levels of HSP90 mRNA and protein in the model group were higher than those in the Xuebijing intervention group (P0.05, P01). The expression level and change trend of HSP90 mRNA and protein in kidney tissue of sepsis mice were similar to those in heart tissue. The heart and kidney tissue of sepsis mice had obvious inflammatory reaction and abnormal tissue structure, while the intervention group maintained a more complete heart and kidney tissue structure, and there was no significant pathological change. The results showed that the expression of HSP90 gene and protein in heart and kidney of sepsis mice increased in varying degrees with the occurrence of sepsis, and increased significantly as early as 2 hours after modeling, indicating that HSP90 factor was involved in the occurrence of sepsis. The development of the course of disease suggests that HSP90 can be listed as one of the early clinical detection and diagnostic indexes of sepsis. Xuebijing intervention can regulate HSP90 factor and effectively improve sepsis symptoms.
【作者单位】：南京医科大学康达学院基础医学部;南通大学实验动物中心;
【基金】：江苏省高校自然科学研究面上项目(16KJD180005) 南京医科大学康达学院科研发展基金项目(KD2015KYJJYB001)
【分类号】：R459.7

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