旋毛虫成虫排泄分泌蛋白抗CLP诱导的小鼠脓毒症的观察
发布时间：2019-07-25 10:22
【摘要】：目的探讨旋毛虫成虫排泄分泌蛋白(AES)对盲肠结扎穿孔术(CLP)诱导的小鼠脓毒症的抑制作用。方法将48只BALB/c小鼠随机分为3组,即假手术对照组(PBS+sham组,A组)、脓毒症组(PBS+CLP组,B组)、AES蛋白治疗组(AES+CLP组,C组)。A组和B组于CLP术后经腹腔注射200μl PBS,C组术后经腹腔注射含25μg AES蛋白的等量PBS。每组随机抽取8只,建模后观察各组小鼠的一般情况及96 h生存率;每组其余8只小鼠于建模后12 h取肝、肺、肾,HE染色观察病理改变;以酶联免疫吸附试验(ELISA)检测小鼠血清中TNF-α、IL-1β、IL-6、IL-10及TGF-β细胞因子水平的变化。结果 3组小鼠96 h生存率差异有统计学意义(χ~2=21.16,P0.05);与A组(100%)相比,B组小鼠生存率(0)降低(P0.05),且肺、肝及肾脏损伤明显加重;经AES治疗后,C组存活率(75%)明显增加(P0.05),小鼠肺脏、肝脏及肾脏病理损伤亦显著缓解。3组小鼠血清中的促炎因子TNF-α(F=27.11,P0.05)、IL-1β(F=18.75,P0.05)及IL-6(F=100.93,P0.05)水平差异均有统计学意义;B组小鼠的促炎因子水平均高于A组(P均0.05);经AES治疗后,C组小鼠上述3种促炎因子水平较B组均显著下降(P均0.05)。3组小鼠血清中的调节性细胞因子IL-10(F=10.88,P0.05)及TGF-β(F=11.37,P0.05)水平差异亦有统计学意义;经AES治疗后,C组小鼠IL-10及TGF-β分泌水平较B组亦明显增加(P0.05)。结论旋毛虫AES对CLP诱导的BALB/c小鼠脓毒症有显著的缓解作用。
[Abstract]:Objective to investigate the inhibitory effect of excretion and secretory protein (AES) of Trichinella spiralis on sepsis induced by cecal ligation and perforation in mice. Methods 48 BALB/c mice were randomly divided into three groups: pseudo-operation control group (PBS sham group (group A), sepsis group (PBS CLP group, group B), AES protein treatment group (AES CLP group, group C). A group and group B after intraabdominal injection of 200 渭 l PBS,C group. The same amount of PBS. containing 25 渭 g AES protein was injected intraperitoneally after CLP operation. Eight mice in each group were randomly selected to observe the general situation and 96 h survival rate of mice in each group, and the pathological changes of liver, lung, kidney and HE were observed at 12 h after modeling, and the levels of TNF- 伪, IL-1 尾, IL-6,IL-10 and TGF- 尾 cytokines in serum were detected by enzyme linked immunosorbent assay (ELISA). Results the 96 h survival rate of mice in group B was significantly lower than that in group A (P < 0.05), and the survival rate in group B was significantly lower than that in group A (P 0.05), and the injury of lung, liver and kidney was significantly worse in group B than that in group A (P < 0.05), and the survival rate in group B was significantly lower than that in group A (P 0.05). After treatment with AES, the survival rate of group C (75%) was significantly increased (P 0.05), and the pathological injury of lung, liver and kidney in mice was also significantly alleviated. The levels of pro-inflammatory factor TNF- 伪 (F 鈮，
本文编号：2519026
[Abstract]:Objective to investigate the inhibitory effect of excretion and secretory protein (AES) of Trichinella spiralis on sepsis induced by cecal ligation and perforation in mice. Methods 48 BALB/c mice were randomly divided into three groups: pseudo-operation control group (PBS sham group (group A), sepsis group (PBS CLP group, group B), AES protein treatment group (AES CLP group, group C). A group and group B after intraabdominal injection of 200 渭 l PBS,C group. The same amount of PBS. containing 25 渭 g AES protein was injected intraperitoneally after CLP operation. Eight mice in each group were randomly selected to observe the general situation and 96 h survival rate of mice in each group, and the pathological changes of liver, lung, kidney and HE were observed at 12 h after modeling, and the levels of TNF- 伪, IL-1 尾, IL-6,IL-10 and TGF- 尾 cytokines in serum were detected by enzyme linked immunosorbent assay (ELISA). Results the 96 h survival rate of mice in group B was significantly lower than that in group A (P < 0.05), and the survival rate in group B was significantly lower than that in group A (P 0.05), and the injury of lung, liver and kidney was significantly worse in group B than that in group A (P < 0.05), and the survival rate in group B was significantly lower than that in group A (P 0.05). After treatment with AES, the survival rate of group C (75%) was significantly increased (P 0.05), and the pathological injury of lung, liver and kidney in mice was also significantly alleviated. The levels of pro-inflammatory factor TNF- 伪 (F 鈮，
本文编号：2519026
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