睾酮对青春期大鼠病理性攻击行为及前额叶皮质PSD-95和GAP-43表达的影响

发布时间：2017-12-31 10:10

本文关键词：睾酮对青春期大鼠病理性攻击行为及前额叶皮质PSD-95和GAP-43表达的影响　出处：《第二军医大学学报》2015年01期 　论文类型：期刊论文

【摘要】：目的探讨去势后睾酮水平对青春期大鼠病理性攻击行为和前额叶皮质突触可塑性的影响。方法将30只出生后21d的雄性SD大鼠随机分为去势组、模型组和对照组。去势组和模型组大鼠采用国际通用应激方法建立病理性攻击模型,持续到青春期。采用居住-入侵实验检测青春期大鼠攻击行为,ELISA法检测睾酮水平,蛋白质印迹分析检测前额叶皮质突触后致密物质95(PSD-95)和生长相关蛋白43(GAP-43)表达水平。结果居住-入侵实验结果显示,去势组攻击潜伏期显著低于模型组和对照组(P0.01,P0.01),屈服后继续攻击次数高于模型组和对照组(P0.01,P0.01)。ELISA检测结果显示,去势组睾酮水平显著低于模型组和对照组(P0.01,P0.01),且睾酮水平与攻击各指标呈中-高度负相关(P0.01)。蛋白质印迹结果显示,去势组前额叶皮质PSD-95和GAP-43表达水平均低于模型组和对照组(P0.01,P0.01)。结论低水平血清睾酮对青春期大鼠前额叶皮质结构可塑性有损伤,可能与其病理性攻击行为相关。
[Abstract]:Objective to investigate the effects of postcastrated testosterone on pathological aggressive behavior and synaptic plasticity in prefrontal cortex of adolescent rats. Methods Thirty male SD rats were randomly divided into castrated group. Model group and control group. Castrated group and model group rats were used to establish pathological attack model by international common stress method, which lasted until puberty. The aggression behavior of adolescent rats was detected by habitation-invasion experiment. Testosterone levels were detected by ELISA. Western blot analysis was used to detect the expression of PSD-95) and GAP-43) in prefrontal cortex. The latency of attack in castration group was significantly lower than that in model group and control group (P 0.01), and the number of continuous attacks after yielding was higher than that in model group and control group (P0.01). The level of testosterone in ovariectomized group was significantly lower than that in model group and control group. The testosterone level was negatively correlated with each index of attack (P 0.01). Western blot analysis showed that testosterone level was negatively correlated with the attack index (P < 0.05). The expression of PSD-95 and GAP-43 in prefrontal cortex of castration group was lower than that of model group and control group (P0.01). Conclusion the low level of serum testosterone may damage the plasticity of prefrontal cortex in puberty rats, which may be related to the pathological aggressive behavior of prefrontal cortex.
【作者单位】：重庆医科大学附属第一医院心理卫生中心;重庆医科大学附属第一医院实验研究中心;
【基金】：重庆市自然科学基金(CSTC,2011jjA10104) 重庆医科大学国家自然科学基金预研项目(NSFYY201111)~~
【分类号】：R749
【正文快照】： Effect of testosterone on pathological aggression behavior and expression of postsynaptics density-95and growthassociated protein-43in prefrontal cortex in puberty ratsL媲Xiao-juan1,ZHANG Yan1,HU Hua1*,CHEN Zhu1,QIN Guang-cheng2,GUI Bei 2,CHEN Li-xue21.D

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