慢性应激抑郁大鼠脑脊液、血清BDNF的变化及艾司西酞普兰抗抑郁剂干预的影响

发布时间：2018-01-31 02:56

  本文关键词： 慢性应激 抑郁症 脑源性神经营养因子 艾司西酞普兰　出处：《东南大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的：基于抑郁症的神经营养假说,本研究通过慢性不可预知温和应激(CUMS)制作抑郁症大鼠模型,对抑郁症大鼠予以艾司西酞普兰抗抑郁剂干预,观察大鼠行为学变化,检测脑脊液、血清脑源性神经营养因子(BDNF)的变化,探讨BDNF与抑郁症的发病关系及艾司西酞普兰干预对BDNF的影响。方法：采用CUMS制作抑郁症大鼠模型,运用强迫游泳实验(forced swimming test,FST)、蔗糖水偏爱实验(sucrose preference test,SPT)、旷场实验(open field test,OFT)和体重增长评估大鼠抑郁行为及艾司西酞普兰抗抑郁剂干预后行为学变化,ELISA法检测抑郁模型大鼠及艾司西酞普兰干预后大鼠脑脊液、血清BDNF的含量。结果：1.建模4w时,与非应激大鼠相比,应激大鼠FST实验中不动时间显著延长[(15.18±8.39)s vs.(73.33±34.71)s],SPT实验中蔗糖消耗百分比显著降低[(86.21±5.54)%vs.(69.68±14.29)%],OFT实验中总路程显著缩短[(1328.65±513.29)cm Vs.(375.71±176.18)cm]、直立次数显著减少[(19.58±6.90)次vs.(3.25±3.25)次],体重增长显著减慢[(376.04±27.27)g vs.(318.97±34.290)g],差异均有统计学意义(p0.01)。2.药物干预4w时,与抑郁模型组相比,抑郁给药组FST实验中不动时间显著缩短[(59.50±31.06)s vs.(20.41±10.67)s],SPT实验中蔗糖消耗百分比显著增加[(48.81±18.90)%vs.(85.27±7.76)%],OFT实验中总路程显著增加[(267.76±255.56)cm vs.(1190.41±306.64)cm],差异均有统计学意义(p0.05)。OFT实验中直立次数比较[(5.17±3.71)次vs.(11.17±1.33)次]、体重比较[(354.73±34.03)g vs.(338.43±40.67)g],差异均无统计学意义(p0.05)。3.药物干预4w时,正常给药组与正常对照组FST实验中不动时间比较[(14.00±6.60)s vs.(18.39±5.37)s]、SPT实验中蔗糖消耗百分比比较[(90.19±4.28)% vs.(94.08±2.66)%]、OFT实验中总路程比较[(1575.06±471.96)cm vs.(1373.38±316.30)cm]、直立次数比较[(14.17±4.45)次vs.(17.67±1.67)次]、体重比较[(437.30±57.24)g vs.(417.03±43.82)g],差异均无统计学意义(p0.05)。4.脑脊液BDNF水平：与正常对照组相比,抑郁模型组CSFBDNF水平显著降低[(1306.30±170.15)pg/ml vs.(1064.69±97.31)pg/ml]；与抑郁模型组相比,抑郁给药组CSF BDNF水平显著升高[(1064.69±97.31)pg/ml vs.(1320.36±189.23)pg/ml],差异均有统计学意义(p0.05)。与正常对照组相比,正常给药组CSF BDNF水平无显著变化[(1306.30±170.15)pg/ml vs.(1416.32±73.48)pg/ml],差异无统计学意义(p0.05)。5.血清BDNF水平：与正常对照组相比,抑郁模型组血清BDNF水平显著降低[(688.14±68.87)pg/ml vs.(374.16±166.73)pg/ml]；与抑郁模型组相比,抑郁给药组血清BDNF水平显著升高[(374.16±166.73)pg/ml vs.(844.17±79.57)pg/ml],差异均有统计学意义(p0.01)。与正常对照组相比,正常给药组血清BDNF水平无显著变化[(688.14±68.87)pg/ml vs.(544.90±97.35)pg/ml],差异无统计学意义(p0.05)。结论：抑郁模型大鼠脑脊液、血清BDNF水平降低,艾司西酞普兰干预能改善抑郁大鼠的抑郁行为,升高抑郁模型大鼠脑脊液、血清BDNF水平,而对正常大鼠无影响。
[Abstract]:Objective: the neurotrophic hypothesis of depression based on the research by chronic unpredictable mild stress (CUMS) model of depression rats to escitalopram depression rats of antidepressant intervention, behavioral changes of the rats were observed, cerebrospinal fluid, serum brain-derived neurotrophic factor (BDNF) changes of BDNF with the onset of depression and escitalopram intervention on BDNF. Methods: the rat model of depression by CUMS, using the forced swimming test (forced swimming, test, FST), sucrose preference test (sucrose preference, test, SPT), open field test (open field, test, OFT) and weight growth evaluation of depression the behavior of rats and the antidepressant escitalopram intervention behavior, detection of depression model rats and escitalopram intervention ELISA method after cerebral spinal fluid, the content of BDNF in serum. Results: 1. 寤烘ā4w鏃，

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