卒中后抑郁大鼠小脑浦肯野细胞形态学改变及神经元凋亡情况研究

发布时间：2018-03-05 07:18

本文选题：卒中　切入点：抑郁　出处：《中国全科医学》2014年15期 　论文类型：期刊论文

【摘要】：目的观察卒中后抑郁(PSD)大鼠小脑浦肯野细胞的形态学改变,并检测神经元的凋亡情况。方法选用健康雄性SD大鼠72只,随机分为假手术组(给予假手术处理)、卒中组(行大脑中动脉闭塞术)、抑郁组〔给予慢性不可预见的温和刺激(CUMS)结合孤养〕和PSD组(大脑中动脉闭塞术后给予CUMS结合孤养)。应激(CUMS)后第1、7、14、21天测量体质量,并行蔗糖水实验和旷野实验(OFT)观察大鼠的行为学变化。造模成功后行尼氏染色观察小脑浦肯野细胞的形态学改变,TUNEL染色检测大鼠小脑浦肯野细胞的凋亡情况,并使用免疫组织化学染色法检测各组大鼠浦肯野细胞激活型半胱氨酸天冬氨酸蛋白酶3(activated-caspase 3)的表达。结果与假手术组比较,卒中组、抑郁组及PSD组大鼠应激后第21天体质量、糖水消耗比例、水平运动及垂直运动均降低(P0.05);与卒中组比较,抑郁组、PSD组大鼠应激后第21天上述指标均降低(P0.05)。与假手术组比较,卒中组、抑郁组、PSD组均有尼氏体减少甚至消失。卒中组、抑郁组、PSD组浦肯野细胞activated-caspase 3阳性细胞数及凋亡率均高于假手术组,抑郁组、PSD组又高于卒中组(P0.05)。结论 PSD大鼠浦肯野细胞存在明显的凋亡损伤,该损伤可能与PSD发病相关。
[Abstract]:Objective To observe the effect of post-stroke depression (PSD) morphological changes of rat cerebellar Purkinje cells, and to detect the apoptotic neurons. Methods 72 healthy male SD rats were randomly divided into sham operation group (sham operation), stroke group (middle cerebral artery occlusion), depression group received mild stimulation chronic unpredictable (CUMS) with solitary and group PSD (middle cerebral artery occlusion treated with CUMS combined with solitary) stress. (CUMS) 1,7,14,21 days after measurement of body weight, sucrose water test and parallel field experiment (OFT) to observe the behavioral changes of rats. Rats were Nigeria Giemsa staining morphological observation of cerebellar Purkinje cells changes, apoptosis of cerebellar Purkinje cells of rats were detected by TUNEL staining, and immunohistochemical staining method to detect the rat Purkinje cells activated caspase 3 (activated-ca Spase 3) expression. Results compared with the sham operation group, stroke group, depression group and PSD group of rats after twenty-first body weight, sugar consumption ratio, horizontal movement and vertical movement decreased (P0.05); compared with the stroke group depression group, PSD group of rats after twenty-first days of above indexes were decreased (P0.05). Compared with the sham operation group, stroke group, depression group and PSD group were decrease or disappearance of Nissl bodies. The stroke group, depression group, PSD group and 3 activated-caspase the number of Purkinje cell apoptosis positive cells were higher than sham operation group, depression group, PSD group was higher than the stroke group (P0.05). Conclusion PSD rat Purkinje cell apoptosis exists obvious damage, the damage may be related to the pathogenesis of PSD.

【作者单位】：辽宁医学院;辽宁医学院附属第一医院神经内科;滨州医学院附属医院;
【基金】：国家自然科学基金(81241050;81371461) 辽宁省自然科学基金(2013022018)
【分类号】：R749.1

【参考文献】