康复训练通过调控海马β淀粉样蛋白表达改善血管性痴呆

发布时间：2018-04-03 17:25

本文选题：康复训练　切入点：血管性痴呆　出处：《南华大学》2013年硕士论文

【摘要】：目的：研究康复训练调控海马β淀粉样蛋白改善血管性痴呆大鼠模型认知功能的机制。方法：将45只大鼠用随机数字表法分为模型组、康复组和假手术组。采用双侧颈总动脉结扎法制作血管性痴呆大鼠模型。康复组大鼠行滚筒、平衡木训练4周。术后4周进行自发活动实验、水迷宫、Y迷宫、新旧物体识别实验进行大鼠行为学评估。采用大鼠海马切片HE染色观察海马形态改变,并行免疫组化染色检测海马β淀粉样蛋白、β分泌酶、胰岛素降解酶表达情况。结果：1.自发活动检测结果:模型组、康复组、假手术组大鼠总活动时间、中央区活动时间、外周活动时间、水平活动距离、中央活动距离和周围活动距离差异均无显著性(p0.05)。 2. Y迷宫实验:模型组Y迷宫实验正确交替比率低于假手术组(p0.05)、康复组Y迷宫实验正确交替率高于模型组(p0.05)，康复组Y迷宫实验正确交替比率低于假手术组(p0.05)。 3.水迷宫实验:模型组水迷宫穿过平台次数低于假手术组、水迷宫实验潜伏期长于假手术组(p0.05)。康复组水迷宫穿过平台次数高于模型组、水迷宫实验潜伏期长于模型组(p0.05)。康复组水迷宫穿过平台次数低于假手术组、水迷宫实验潜伏期长于假手术组(p0.05)。 4.新旧物体识别实验:模型组新旧物体识别实验分辨指数低于假手术组(p0.05)。模型组新旧物体识别实验分辨指数低于康复组(p0.05)。康复组新旧物体识别实验分辨指数低于假手术组(p0.05)。 5. HE染色:低倍镜下模型组大鼠海马DG区细胞数目少,排列稀疏,康复组大鼠海马DG区细胞数较假手组大鼠稀疏但较模型组多。高倍镜下,模型组海马DG区空泡细胞多,康复组亦可见少量空泡细胞,假手术组未见空泡细胞。 6. β-淀粉样蛋白免疫组化表达结果:模型组海马β淀粉样蛋白表达高于假手术组(p0.05)。康复组海马β淀粉样蛋白表达低于模型组(p0.05)。康复组海马β淀粉样蛋白表达高于假手术组(p0.05)。 7.各组大鼠海马β分泌酶免疫组化表达结果:模型组海马β分泌酶表达高于假手术组(p0.05)。康复组海马β分泌酶表达低于模型组(p0.05)。康复组海马β分泌酶表达高于假手术组(p0.05)。康复组海马β分泌酶表达高于假手术组(p0.05)。 8.各组大鼠海马胰岛素降解酶免疫组化表达结果:模型组海马胰岛素降解酶表达低于假手术组(p0.05)。康复组海马胰岛素降解酶表达高于模型组(p0.05)。康复组海马胰岛素降解酶表达低于假手术组(p0.05)。结论：康复训练可改善血管性痴呆大鼠认知功能，，这种保护作用可能通过下调BACE及上调IDE在VD大鼠海马DG区的表达来减少Aβ在海马DG区的表达。
[Abstract]:Aim: to study the mechanism of rehabilitation training on the improvement of cognitive function of vascular dementia rats by 尾 -amyloid in hippocampus.Methods: 45 rats were randomly divided into three groups: model group, rehabilitation group and sham operation group.Vascular dementia rat model was established by bilateral common carotid artery ligation.Rats in rehabilitation group were trained with roller and balance beam for 4 weeks.Spontaneous activity test, water maze and Y maze were performed 4 weeks after operation.The changes of hippocampal morphology were observed by HE staining and the expression of 尾 -amyloid, 尾 -secretase and insulin-degrading enzyme in hippocampus were detected by immunohistochemical staining.The result is 1: 1.Results of spontaneous activity detection: there was no significant difference in total activity time, central area activity time, peripheral activity time, horizontal activity distance, central activity distance and peripheral activity distance between model group, rehabilitation group and sham operation group.2.Y maze experiment: the correct alternating rate of Y maze test in model group was lower than that in sham operation group, the correct alternating rate of Y maze test in rehabilitation group was higher than that in model group, and the correct alternation rate of Y maze experiment in rehabilitation group was lower than that in sham operation group.3.Water maze test: the number of water labyrinth passing through the platform in the model group was lower than that in the sham operation group, and the incubation period of the water maze test was longer than that of the sham operation group.The times of water labyrinth passing through the platform in rehabilitation group were higher than that in model group, and the latency of water maze test was longer than that of model group (P 0.05).The times of water labyrinth passing through the platform in rehabilitation group were lower than that in sham operation group, and the latency of water maze test was longer than that of sham operation group.4.New and old object recognition experiment: the resolution index of model group was lower than that of sham-operation group (P 0.05).The experimental resolution index of old and new object recognition in model group was lower than that in rehabilitation group (P 0.05).The experimental resolution index of old and new object recognition in rehabilitation group was lower than that in sham operation group (P 0.05).5.He staining: the number of DG cells in the hippocampus of the model group was less and the number of the cells in the hippocampal DG region of the rehabilitation group was less than that of the artificial hand group but more than that of the model group.Under high power microscope, there were more vacuoles in DG area of hippocampus in model group, a small number of vacuoles in rehabilitation group, and no vacuole cells in sham operation group.6. Expression of 尾 -amyloid protein: the expression of 尾 -amyloid protein in hippocampus of model group was higher than that of sham operation group (P 0.05).The expression of 尾 -amyloid protein in hippocampus of rehabilitation group was lower than that of model group.The expression of 尾 -amyloid protein in hippocampus of rehabilitation group was higher than that of sham operation group.7.The expression of 尾 -secretase in hippocampus of model group was higher than that of sham operation group (P 0.05).The expression of 尾 -secretase in hippocampus of rehabilitation group was lower than that of model group.The expression of 尾 -secretase in hippocampus of rehabilitation group was higher than that of sham operation group (P 0.05).The expression of 尾 -secretase in hippocampus of rehabilitation group was higher than that of sham operation group (P 0.05).8.The expression of insulinase in hippocampus of the model group was lower than that of the sham-operated group (P 0.05).The expression of insulin-degrading enzyme in hippocampus of rehabilitation group was higher than that of model group.The expression of insulinase in hippocampus of rehabilitation group was lower than that of sham operation group (P 0.05).Conclusion: rehabilitation training can improve the cognitive function of vascular dementia rats. This protective effect may reduce the expression of A 尾 in the DG region of hippocampus by down-regulating BACE and up-regulating the expression of IDE in the DG region of hippocampus of VD rats.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.13

【共引文献】