脑卒中后抑郁大鼠丘脑脑源性神经营养因子及酪氨酸激酶受体B蛋白的表达变化

发布时间：2018-05-02 09:45

本文选题：卒中 + 抑郁　；参考：《中华老年心脑血管病杂志》2014年05期

【摘要】：目的探讨脑卒中后抑郁(PSD)大鼠丘脑脑源性神经营养因子(BDNF)及其高亲和力受体酪氨酸激酶受体B(TrkB)蛋白的表达变化,及其在PSD发病机制中的作用和意义。方法选择成年SD大鼠24只,随机分为对照组、抑郁组、脑卒中组和模型组,每组6只。利用线栓法建立局灶性脑缺血大鼠模型,加以慢性不可预见的中等应激刺激和孤养方法造成PSD动物模型,应用免疫组织化学方法检测各组大鼠造模后第29天丘脑BDNF和TrkB免疫阳性细胞数的表达变化。结果模型组BDNF阳性细胞表达较对照组和抑郁组明显减少,差异有统计学意义(P0.05);模型组TrkB阳性细胞表达较对照组、脑卒中组和抑郁组明显减少,差异有统计学意义[(9.00±2.12)个vs(41.22±11.91)个、(17.22±5.76)个和(33.67±8.32)个,P0.01]。结论 PSD大鼠丘脑BDNF及其高亲和力受体TrkB蛋白表达减少可能与PSD发病机制有相关性。
[Abstract]:Objective to investigate the expression of brain-derived neurotrophic factor (BDNF) and its high affinity receptor tyrosine kinase receptor (BPT-TrkB) in the thalamus of rats with post-stroke depression (PSD) and its role and significance in the pathogenesis of PSD. Methods Twenty-four adult SD rats were randomly divided into control group, depression group, stroke group and model group with 6 rats in each group. The rat model of focal cerebral ischemia was established by the method of thread embolization, and the animal model of PSD was induced by chronic unpredictable medium stress stimulation and solitary rearing. The expression of BDNF and TrkB immunoreactive cells in thalamus of rats in each group were detected by immunohistochemical method on the 29th day after modeling. Results the expression of BDNF positive cells in model group was significantly lower than that in control group and depression group, the difference was statistically significant (P 0.05), and the expression of TrkB positive cells in model group was significantly lower than that in control group, stroke group and depression group. The difference was statistically significant (9.00 卤2.12) vs(41.22 卤11.91) (17.22 卤5.76) and 33.67 卤8.32 (P0.01). Conclusion the decreased expression of BDNF and its high affinity receptor TrkB protein in thalamus of PSD rats may be related to the pathogenesis of PSD.
【作者单位】：大理学院附属医院神经内科;大理州公安局刑事科学研究所;
【基金】：云南省社会发展科技计划项目(2009ZC126M)
【分类号】：R749.1

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