氢饱和盐水对阿尔茨海默病模型大鼠氧化应激损伤的影响

发布时间：2018-05-18 22:32

本文选题：阿尔茨海默病 + 氢饱和盐水　；参考：《中国老年学杂志》2014年10期

【摘要】：目的探索氢饱和盐水对阿尔茨海默病(AD)模型大鼠氧化应激的影响。方法选用280~320 g Sprague-Dawley(SD)大鼠,随机分为模型组、假手术组、氢饱和盐水治疗组;利用立体定向技术侧脑室注射Aβ1~42建立AD模型大鼠,腹腔注射氢饱和盐水。给药14 d后采用生物化学法检测各组大鼠的脑海马组织匀浆的MDA含量,利用免疫组织化学法检测各组大鼠脑海马组织氧化应激标志物HNE及8-OH-d G蛋白的表达。结果模型组大鼠脑组织MDA含量为(6.65±1.07)nmol/mgprotein,与假手术组相比显著升高(P=0.000),而氢饱和盐水治疗组大鼠脑组织的MDA含量较模型组大鼠显著降低(P=0.016)。模型组大鼠海马齿状回显现较多的HNE阳性细胞,假手术组大鼠鲜见HNE阳性细胞,而氢饱和盐水治疗组大鼠海马齿状回HNE阳性细胞较模型组大鼠显著减少(P=0.011)。海马齿状回呈现棕黄色8-OH-d G染色阳性细胞,与假手术组大鼠相比,模型组大鼠海马齿状回出现明显增多的8-OH-d G染色阳性细胞(P=0.000),而经氢饱和盐水治疗后的模型大鼠海马齿状回8-OH-d G染色阳性细胞明显减少(P=0.000)。顶叶皮层呈现棕黄色8-OH-d G染色阳性细胞,与假手术组大鼠相比,模型组大鼠顶叶皮层出现明显增多的8-OH-d G染色阳性细胞(P=0.000),而经氢饱和盐水治疗后的模型大鼠顶叶皮层8-OH-d G染色阳性细胞明显减少(P=0.000)。结论氢饱和盐水可显著减少Aβ诱导的氧化应激损伤蛋白及核酸标志物的表达。
[Abstract]:Objective to investigate the effect of hydrogen saturated saline on oxidative stress in rats with Alzheimer's disease (AD). Methods SD rats were randomly divided into three groups: model group, sham-operated group and hydrogen saturated saline treatment group, AD model rats were induced by intracerebroventricular injection of A 尾 1N 42 by stereotactic technique and intraperitoneally injected with hydrogen saturated saline. After 14 days of administration, the content of MDA in brain tissue homogenate of rats in each group was detected by biochemical method, and the expression of HNE and 8-OH-d G protein in brain tissue of each group was detected by immunohistochemical method. Results the content of MDA in the brain tissue of the model group was 6.65 卤1.07nmol / mg protein, which was significantly higher than that of the sham-operated group, while the content of MDA in the brain tissue of the model group was significantly lower than that of the model group. There were more HNE positive cells in the dentate gyrus of the model group, but few HNE positive cells in the sham-operated group, while the HNE positive cells in the dentate gyrus of the rats in the hydrogen saturated saline group were significantly lower than those in the model group. The dentate gyrus of the sea horse showed brown 8-OH-d G staining positive cells, compared with the sham-operated rats. In the model group, the number of 8-OH-d G staining positive cells in dentate gyrus was significantly increased, while the 8-OH-d G staining positive cells in the dentate gyrus of the model rats treated with hydrogen saturated saline significantly decreased. The parietal cortex showed brown 8-OH-d G staining positive cells, compared with sham-operated rats. In the model group, the number of 8-OH-d G staining positive cells in the parietal cortex was significantly increased, while that in the parietal cortex of the model rats treated with hydrogen saturated saline decreased significantly. Conclusion hydrogen saturated saline can significantly reduce the expression of A 尾 -induced oxidative stress damage proteins and nucleic acid markers.
【作者单位】：辽宁医学院附属第一医院神经内科;辽宁医学院药理教研室;
【基金】：国家自然科学基金(No.81201037) 辽宁省博士启动基金计划(No.20131068) 辽宁医学院博士启动项目(No.Y2012B015)
【分类号】：R749.16

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