海马内注射Aβ1-42寡聚体影响大鼠焦虑、抑郁行为及突触可塑性

发布时间：2018-05-25 21:35

本文选题：淀粉样β蛋白 + 行为脱抑制　；参考：《神经解剖学杂志》2014年04期

【摘要】：目的:探讨淀粉样β蛋白(Aβ)对大鼠焦虑和抑郁行为的影响及其可能的突触机制。方法:将SD大鼠随机分为对照组(n=10)和Aβ1-42注射组(n=10)。通过联合采用高架十字迷宫、强迫游泳及电生理实验技术,以动物在迷宫开臂和闭臂中停留时间百分比、在强迫游泳中的不动时间以及在体海马场兴奋性突触后电位(fEPSP)的幅度作为主要观察指标,系统研究了海马内注射Aβ1-42寡聚体对大鼠焦虑、抑郁行为及海马突触可塑性的影响。结果:(1)Aβ1-42组大鼠在高架十字迷宫开臂中所停留的时间百分比明显大于正常组(P0.01),在闭臂中停留的时间百分比则明显小于对照组(P0.01);(2)Aβ1-42组大鼠在强迫游泳测试中"不动"时间较对照组明显增加(P0.01),"游泳"时间则明显减少(P0.05);(3)高频刺激(HFS)后Aβ1-42组大鼠的海马LTP受到明显压抑,HFS后即刻以及HFS后30 min和60 min时fEPSP平均幅度均明显下降(P0.05或P0.01)。结论:海马内注射Aβ可导致大鼠行为脱抑制、引起抑郁样行为并伤害突触可塑性,提示海马LTP的异常不仅影响学习记忆功能,可能也与焦虑和抑郁等精神行为异常的发生有关。
[Abstract]:Aim: to investigate the effect of amyloid 尾 protein A 尾 on anxiety and depression in rats and its possible synaptic mechanism. Methods: SD rats were randomly divided into control group (n = 10) and A 尾 1-42 injection group (n = 10). By combined use of elevated cross labyrinth, forced swimming and electrophysiological experiments, the percentage of time animals stayed in the labyrinth's open and closed arms, The effects of intrahippocampal injection of A 尾 1-42 oligomer on anxiety, depression behavior and hippocampal synaptic plasticity in rats were systematically studied by using the immobility time in forced swimming and the amplitude of excitatory postsynaptic potential (fEPSP) in the hippocampus as the main indexes. Results the percentage of staying time in the open arm of the cross labyrinth was significantly higher in the group 1: 1 than in the normal group, and the percentage in the closed arm was significantly lower than that in the control group when the rats in the group A 尾 1-42 were "stationary" in the forced swimming test. The percentage of the retention time in the group A 尾 1-42 was significantly lower than that in the control group in the open arm of the cross labyrinth, and the percentage of the retention in the closed arm was significantly lower than that in the control group. Compared with the control group, the time of "swimming" significantly decreased the mean amplitude of fEPSP in the hippocampus of A 尾 1-42 rats after high frequency stimulation (P 0.05) and the mean amplitude of fEPSP decreased significantly at 30 min and 60 min after HFS in group A 尾 1-42. Conclusion: intrahippocampal injection of A 尾 can induce depressive behavior, depression-like behavior and damage synaptic plasticity in rats. It is suggested that abnormal LTP in hippocampus not only affects learning and memory function, but also may be related to the occurrence of mental and behavioral disorders such as anxiety and depression.
【作者单位】：山西医科大学生理学系;
【基金】：国家自然科学基金(31271201) 山西省自然科学基金(2010011049-3)
【分类号】：R749.4;R749.72

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