金属硫蛋白3对快速老化痴呆模型小鼠海马组织学变化的影响

发布时间：2018-06-05 08:05

本文选题：金属硫蛋白 + 快速老化痴呆模型小鼠　；参考：《中国老年学杂志》2014年09期

【摘要】：目的研究金属硫蛋白(MT)3对快速老化痴呆模型小鼠(SAMP8)海马结构CA1区神经元组织学变化的影响。方法给小鼠不同浓度MT3及盐水腹腔注射28 d,处死小鼠后应用光镜和电镜观察制备标本海马结构神经元的变化。结果快速老化小鼠对照小鼠(SAMR1)海马CA1区锥体细胞排列较整齐、均匀,胞膜完整,染色质分布均匀,细胞核呈圆形或椭圆形,核仁清楚,细胞器清晰可见。SAMP8组海马CA1区可见锥体细胞稀疏、排列紊乱,大部分神经元肿胀、空化,出现凋亡细胞,很多胞核固缩深染,胶质细胞增多,有较多脂褐素沉积。低、中、高浓度MT3组海马CA1区锥体细胞较空白对照组结构较清晰,排列较整齐均匀。神经元核基质呈轻度空化改变,胞质内都可见线粒体、粗面内质网及脂褐素,胶质细胞减少。高浓度MT3组的超微结构最接近于SAMR1组,但仍处于老化状态。结论 SAMP8海马有组织结构和超微结构的改变。MT3对SAMP8海马锥体细胞有保护的作用。
[Abstract]:Objective to study the effect of metallothionein (MTX) 3 on the histological changes of hippocampal CA1 neurons in mice with rapid aging dementia. Methods mice with different concentrations of MT3 and saline were injected intraperitoneally for 28 days. The changes of hippocampal neurons were observed by light microscope and electron microscope. Results the hippocampal CA1 pyramidal cells were arranged neatly and evenly, the membrane was intact, the chromatin was distributed evenly, the nucleus was round or elliptical, and the nucleoli were clear. In group .SAMP8, pyramidal cells were sparse and disordered, most neurons were swollen, cavitated, apoptotic cells appeared, many nuclei were pyknosis, glial cells were increased and lipofuscin was deposited. The structure of hippocampal CA1 pyramidal cells in the low, middle and high concentration MT3 group was clearer than that in the blank control group. The nucleus matrix of neurons showed slight cavitation, mitochondria, rough endoplasmic reticulum, lipofuscin and glia decreased in cytoplasm. The ultrastructure of high concentration MT3 group was closest to that of SAMR1 group, but it was still aging. Conclusion the changes of SAMP8 hippocampal tissue and ultrastructure. MT3 has protective effect on SAMP8 hippocampal pyramidal cells.
【作者单位】：汕头市中心医院;中山大学附属汕头医院神经内科;
【基金】：广东省医学科研基金立项(粤卫〔2011〕74号)
【分类号】：R749.16

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