氟西汀对人嗅黏膜神经干细胞增殖与神经发生的影响

发布时间：2018-06-06 16:58

本文选题：成人嗅黏膜 + 神经干细胞　；参考：《重庆医科大学》2017年硕士论文

【摘要】：目的探索以氟西汀为代表的抗抑郁药对人嗅黏膜神经干细胞增殖及神经发生的影响。为抗抑郁治疗能够促进神经发生这一理论增添新的证据。方法采用组织块培养法获取人嗅黏膜细胞后,用神经球培养法纯化扩增神经干细胞,采用细胞免疫荧光染色验证神经干细胞及由其分化而来的神经细胞的特异性。分别采用CCK-8、Edu技术检测抗抑郁药对嗅黏膜神经干细胞活性及增殖的影响;采用蛋白质分子印记技术检测抗抑郁药对其神经发生的影响。结果1.Nestin(神经干细胞标志蛋白)细胞荧光染色阳性;由其分化而来的细胞TUJ1(神经细胞特异标志物)细胞荧光染色阳性。神经干细胞培养成功。2.CCK-8实验显示一定浓度的氟西汀可加强嗅黏膜神经干细胞的活力;Edu实验显示一定浓度的氟西汀可促进神经干细胞增殖。3.蛋白质分子印迹实验显示一定浓度氟西汀处理的嗅黏膜神经干细胞,其TUJ1的表达量较对照组显著上升,即氟西汀可以促进干细胞分化成神经细胞。结论1.人嗅黏膜神经干细胞原代培养成功且其可分化为神经细胞。2.抗抑郁治疗可促进神经干细胞的增殖及向神经细胞分化,有可能为抗抑郁药物治疗的机制之一。
[Abstract]:Objective to investigate the effects of fluoxetine on the proliferation and neurogenesis of neural stem cells in human olfactory mucosa. Add new evidence to the theory that antidepressant therapy promotes neurogenesis. Methods Human olfactory mucosal cells were obtained by tissue mass culture. The neural stem cells were purified and amplified by the method of neurosphere culture. The specificity of neural stem cells and the nerve cells derived from them were tested by immunofluorescence staining. CCK-8 Edu technique was used to detect the effects of antidepressants on the activity and proliferation of neural stem cells in olfactory mucosa, and protein molecular imprinting technique was used to detect the effects of antidepressants on neurogenesis. Results 1. Nestin (neural stem cell marker protein) cells were positive for fluorescence staining, and TUJ1 (neuronal specific marker) cells from Nestin (neural stem cell marker protein) cells were positive for fluorescence staining. CCK-8 experiment showed that a certain concentration of fluoxetine could enhance the activity of neural stem cells in olfactory mucosa. Edu experiment showed that a certain concentration of fluoxetine could promote the proliferation of neural stem cells. Western blot analysis showed that the expression of TUJ1 in olfactory mucosal neural stem cells treated with fluoxetine at a certain concentration was significantly higher than that in control group, that is, fluoxetine could promote the differentiation of stem cells into neural cells. Conclusion 1. Human olfactory mucosal neural stem cells were successfully cultured and differentiated into neural cells. 2. Antidepressant therapy can promote the proliferation and differentiation of neural stem cells, which may be one of the mechanisms of antidepressant therapy.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749.4

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