孤独症大鼠海马自噬相关蛋白的表达

发布时间：2018-07-28 09:47

【摘要】：目的探讨细胞自噬在孤独症发病中的作用。方法健康繁殖期Wistar雌鼠20只(250~260g),雄鼠10只(280~290g),按2∶1比例合笼过夜,次日早上阴道涂片发现精子计为孕1d。在其孕12.5d时,10只雌鼠腹腔注射丙戊酸钠,其子代为孤独症模型组;10只注射同等剂量生理盐水,其子代为正常组。通过比较自梳理实验、三箱实验验证孤独症模型是否成功;Western blotting方法对比正常组与孤独症模型组大鼠生后42d(P42)海马自噬相关蛋白LC3-Ⅱ、Beclin 1和P62表达的变化。结果 1.成功建立孤独症动物模型,与正常组比较,自梳理实验显示,模型组理毛时间较正常显著增加(P0.05),三箱实验结果显示,模型组大鼠交互能力障碍、缺乏对新鲜事物的偏好性;2.与正常组相比较,模型组大鼠P42d海马自噬相关蛋白LC3-Ⅱ表达显著降低(P0.05),Beclin 1表达显著降低(P0.05),P62表达显著升高(P0.05)。结论孤独症大鼠P42d海马自噬活性降低,提示增强自噬可能是一个潜在的治疗策略。
[Abstract]:Objective to investigate the role of autophagy in the pathogenesis of autism. Methods 20 Wistar female rats (250 ~ 260g) and 10 males (280-290g) were used to spend the night in a cage in proportion to 2:1. The next morning vaginal smear showed that the sperm was pregnant for 1 day. On the 12th day of pregnancy, 10 female rats were injected intraperitoneally with sodium valproate, 10 rats in the autism model group were injected with the same dose of normal saline, and their children were the normal group. By comparing self-combing experiment and three-box experiment, we verified whether the autistic model was successful or not and whether the expression of LC3- 鈪，

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