妊娠期慢性应激对不同性别子代抑郁的影响

发布时间：2018-08-18 16:05

【摘要】：目的探讨妊娠期慢性应激对不同性别子代小鼠抑郁程度的影响及其可能机制。方法实验分为妊娠期正常处理-子代正常处理(Control)组、妊娠期慢性应激-子代正常处理(CPS)组、妊娠期正常处理-子代慢性应激(COS)组、妊娠期慢性应激-子代慢性应激(COS+CPS)组。通过强迫游泳实验观察子代小鼠的抑郁程度;HE染色观察子代小鼠海马CA 3区神经元病理组织形态变化;Western blot法检测小鼠海马中雷帕霉素靶蛋白(m TOR)的表达量;ELISA法检测小鼠血清中促肾上腺皮质激素释放激素(CRH)的含量。结果与Control组相比,子代雄鼠COS组不动时间延长(P0.05),海马CA 3区神经元细胞损伤的数目明显增加,神经元排列疏松、紊乱,核固缩、浓染;海马组织中m TOR表达量减少(P0.05);血清CRH浓度升高(P0.05);子代雌鼠COS组不动时间延长(P0.05),海马组织中m TOR表达量减少(P0.05),其余指标未呈现类似结果。与COS组相比,子代雄鼠COS+CPS组不动时间延长(P0.05),海马CA 3区神经元进一步损伤,海马组织中m TOR的表达量减少(P0.05),血清中CRH浓度升高(P0.05);子代雌鼠COS+CPS组的上述指标亦未进一步改变。结论妊娠期慢性应激进一步加剧慢性应激所致的子代雄鼠抑郁,其机制可能与其升高小鼠血清CRH水平,抑制m TOR的表达,最终引起海马CA 3区神经元损伤有关。妊娠期慢性应激未能进一步加剧子代雌鼠应激所致的抑郁程度。
[Abstract]:Objective to investigate the effect of chronic stress during pregnancy on the depression degree of mice of different sexes and its possible mechanism. Methods the experiment was divided into three groups: (Control) group, (CPS) group, (COS) group, (COS CPS) group. Observation of the degree of depression in offspring mice by forced swimming and observation of histopathological changes of hippocampal neurons in hippocampal CA _ 3 region by HE staining; Western blot method for detecting the expression of rapamycin target protein (m TOR) in hippocampus of mice; Elisa method for detection of rapamycin target protein (m TOR) in hippocampus of mice The content of corticotropin releasing hormone (CRH) in serum of mice was measured. Results compared with Control group, the immobility time of COS group was prolonged (P0.05), the number of neuronal cell injury in hippocampal CA 3 area was significantly increased, the neurons were loosely arranged, disordered, nuclear pyknosis and staining were observed. The expression of m TOR in hippocampus was decreased (P0.05); the concentration of serum CRH was increased (P0.05); the immobility time of COS group was prolonged (P0.05), and the expression of m TOR in hippocampus was decreased (P0.05). The other indexes did not show similar results. Compared with COS group, the immobility time of male COS CPS group was prolonged (P0.05), the hippocampal CA3 neurons were further damaged, the expression of m TOR in hippocampus was decreased (P0.05), the serum CRH concentration was increased (P0.05), and the above indexes of COS CPS group were not further changed. Conclusion chronic stress during pregnancy further exacerbates the depression in offspring induced by chronic stress, which may be related to the increase of serum CRH level, the inhibition of m TOR expression, and eventually the neuronal damage in hippocampal CA 3 area. Chronic stress during pregnancy did not further aggravate the depression caused by stress in female offspring.
【作者单位】：安徽医科大学公共卫生学院营养与食品卫生学系;安徽医科大学公共卫生学院卫生检验与检疫学系;
【基金】：安徽省高校省级自然科学研究重点项目(编号:KJ2016A354)
【分类号】：R749.4

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