脑源性神经营养因子含量和基因多态性与精神分裂症的相关分析
发布时间：2018-09-06 14:59
【摘要】：背景与目的： 精神分裂症,英文名称为schizophrenia,属于一种较为常见的精神病。有资料表明,世界范围内,精神分裂症患者的终身患病率约为3.8‰～8.4‰。资料显示,我国有很多人处于不同程度的精神分裂状态。一旦患上精神分裂症,将会在不同程度影响患者的生活质量和患者的身心健康。目前认为遗传因素、内分泌因素、躯体生物学因素、脑结构变化因素等多方面的原因等均可引发精神分裂症。有学者研究表明,精神分裂症的发病和神经发育异常有着密切的关系,在所有因子中,脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)是其中最为关键的因子之一。为此,本研究拟通过分析精神分裂症患者的临床表型与脑源性神经营养因子基因多态性、以及血清BDNF浓度的相关性,为精神分裂症发病机制的探索提供实验资料。 研究方法： 选取商丘市精神病医院住院部的456例病例较为完整的精神分裂症患者作为研究对象,同时选取114例正常人作为实验的对照组。并由同一组经验丰富的精神科主治医师对实验组患者进行PANSS量表与PSP量表评分；选取实验组不同临床诊断分型患者PSP评分分析不同精神分裂症临床亚型对患者社会认知和社会功能的影响；采用酶联免疫吸附(ELISA)法测定2组标本血清中BDNF浓度；采用TaqMan法检测BDNF基因单核苷酸多态性(SNP) rs6265和rs11030101位点。分析精神分裂症患者性别、年龄与血清BDNF浓度的相关性；分析精神分裂症不同临床诊断分型与患者血清BDNF浓度的相关性；分析不同(SNPs rs6265和rs11030101)基因型、等位基因型分布和联合基因型频率分布与精神分裂症的相关性,与患者PANSS和PSP的相关性。 研究结果： 实验组患者PANSS量表评定Ⅰ型与Ⅱ型患者男女比例、平均病程及平均年龄比较差异无统计学意义(P0.05)；实验组患者PSP量表平均评分为(46.04±16.02),表明患者在发病时社会认知和社会功能已经受到了不同程度的损害；实验组患者不同临床分型PSP评分比较差异无统计学意义(P0.05)；实验组患者血清BDNF低于对照组，，差异具有显著性(P0.05)；2组患者不同性别血清BDNF浓度比较差异无统计学意义(P0.05)；2组患者不同年龄血清BDNF浓度比较差异也无统计学意义(P0.05)；实验组患者不同的临床诊断分型患者血清BDNF浓度比较无统计学差异(P0.05)；实验组患者的血清BDNF浓度、PSP量表评分和PANSS量表评分正交相关分析表明：患者的PSP评分与PANSS评分之间呈负相关,差异具有显著性(P0.05),其余各指标间无统计学意义相关性(P0.05)；2组SNPs rs6265和rs11030101基因型及等位基因比较差异无统计学意义(P0.05)；2组患者男性患者与女性患者SNPs rs6265和rs11030101基因型、等位基因频率分布比较差异无统计学意义(P0.05)；2组患者SNPs rs6265和rs11030101基因型、等位基因频率分布男女比例比较差异均无统计学意义(P0.05)；2组患者联合基因型(SNPs rs6265rs11030101)频率分布差异无统计学意义(P0.0.5)；实验组患者不同临床诊断分型SNPs rs6265和rs11030101基因型及等位基因型分布差异无统计学意义(P0.05)；不同临床诊断分型患者SNPs rs6265和rs11030101基因型及等位基因分布比较,差异无统计学意义(P0.05)；实验组SNPs rs6265和rs11030101不同基因型患者PANSS评分比较差异无统计学意义(P0.05)；SNPs rs6265不同基因型患者PSP评分比较差异无统计学意义(P0.05), SNPs rs11030101不同基因型患者PSP评分G/A最高、A/A最低,差异具有显著性(P0.05)；实验组患者不同SNPs rs6265和rs11030101基因型血清BDNF浓度比较差异无统计学意义(P0.05)。 结论： 精神分裂症患者血清BDNF水平低于正常人。精神分裂症患者SNPs rs11030101位点不同基因型可能对患者个人和社会功能评分产生影响,其中G/A型患者影响最高,A/A型患者影响最低。
[Abstract]:Background and purpose:
Schizophrenia is a common psychiatric disorder. Data show that the lifetime prevalence of schizophrenia is about 3.8 ~8.4. Data show that many people in China are in different degrees of schizophrenia. At present, it is believed that genetic factors, endocrine factors, somatic biological factors, brain structural changes and other factors can cause schizophrenia. Some scholars have shown that the onset of schizophrenia and neurodevelopmental abnormalities have a close relationship, in all the factors, brain origin. Sexual neurotrophic factor (BDNF) is one of the most important factors in schizophrenia. Therefore, this study intends to analyze the correlation between clinical phenotype and brain-derived neurotrophic factor gene polymorphism, and serum BDNF concentration in schizophrenia, so as to provide an experiment for exploring the pathogenesis of schizophrenia. Information.
Research methods:
A total of 456 schizophrenic patients in the inpatient department of Shangqiu Psychiatric Hospital were selected as the subjects and 114 normal persons as the control group. The PSP score of the severed schizophrenia patients was used to analyze the influence of different clinical subtypes on their social cognitive and social functions; the serum BDNF concentration was determined by enzyme-linked immunosorbent assay (ELISA); the single nucleotide polymorphism (SNP) rs6265 and rs11030101 of BDNF gene were detected by TaqMan method. In addition, the correlation between age and serum BDNF concentration was analyzed; the correlation between different clinical diagnostic types and serum BDNF concentration was analyzed; the correlation between different genotypes (SNPs rs6265 and rs11030101), allele distribution and frequency distribution of combined genotypes and schizophrenia, and the correlation between PANSS and PSP were analyzed.
Research findings:
There was no significant difference in the average course of disease and age between the two groups (P 0.05). The average score of PSP in the experimental group was (46.04 +16.02), indicating that the patients'social cognition and social function had been impaired in different degrees. There was no significant difference in clinical classification PSP score (P 0.05); serum BDNF in experimental group was lower than that in control group, the difference was significant (P 0.05); there was no significant difference in serum BDNF concentration between the two groups (P 0.05); there was no significant difference in serum BDNF concentration between the two groups at different ages (P 0.05). There was no significant difference in serum BDNF concentration among patients with different clinical diagnosis types (P 0.05). The orthogonal correlation analysis of serum BDNF concentration, PSP score and PANSS score showed that there was a negative correlation between PSP score and PANSS score, and the difference was significant (P 0.05). Significance correlation (P 0.05); There was no significant difference in genotype and allele between the two groups (P 0.05); SNPs rs6265 and rs11030101 genotypes and allele frequencies between male and female patients in the two groups had no significant difference (P 0.05); SNPs rs6265 and rs11030101 genotypes and allele frequencies in the two groups had no significant difference (P 0.05). There was no significant difference in the frequency distribution of alleles between men and women (P 0.05); there was no significant difference in the frequency distribution of combined genotypes (SNPs rs6265 rs11030101) between the two groups (P 0.0.5); there was no significant difference in the distribution of genotypes and alleles of SNPs rs6265 and rs11030101 between the two groups (P 0.05). There was no significant difference in the genotype and allele distribution of SNPs rs6265 and rs11030101 among the patients with different clinical diagnosis types (P 0.05); there was no significant difference in the PANSS score between the patients with different genotypes of SNPs rs6265 and rs11030101 in the experimental group (P 0.05); there was no significant difference in the PSP score among the patients with different genotypes of SNPs rs6265. Significance (P 0.05), different genotypes of SNPs rs11030101 patients with the highest PSP score G/A, the lowest A/A, the difference was significant (P 0.05); experimental group of different SNPs rs6265 and rs11030101 genotype serum BDNF concentration difference was not statistically significant (P 0.05).
Conclusion:
The level of serum BDNF in schizophrenic patients was lower than that in normal subjects. Different genotypes at SNPs rs11030101 locus may have an impact on individual and social function scores of patients with schizophrenia, of which G/A type had the highest impact and A/A type had the lowest impact.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.3
本文编号：2226707
[Abstract]:Background and purpose:
Schizophrenia is a common psychiatric disorder. Data show that the lifetime prevalence of schizophrenia is about 3.8 ~8.4. Data show that many people in China are in different degrees of schizophrenia. At present, it is believed that genetic factors, endocrine factors, somatic biological factors, brain structural changes and other factors can cause schizophrenia. Some scholars have shown that the onset of schizophrenia and neurodevelopmental abnormalities have a close relationship, in all the factors, brain origin. Sexual neurotrophic factor (BDNF) is one of the most important factors in schizophrenia. Therefore, this study intends to analyze the correlation between clinical phenotype and brain-derived neurotrophic factor gene polymorphism, and serum BDNF concentration in schizophrenia, so as to provide an experiment for exploring the pathogenesis of schizophrenia. Information.
Research methods:
A total of 456 schizophrenic patients in the inpatient department of Shangqiu Psychiatric Hospital were selected as the subjects and 114 normal persons as the control group. The PSP score of the severed schizophrenia patients was used to analyze the influence of different clinical subtypes on their social cognitive and social functions; the serum BDNF concentration was determined by enzyme-linked immunosorbent assay (ELISA); the single nucleotide polymorphism (SNP) rs6265 and rs11030101 of BDNF gene were detected by TaqMan method. In addition, the correlation between age and serum BDNF concentration was analyzed; the correlation between different clinical diagnostic types and serum BDNF concentration was analyzed; the correlation between different genotypes (SNPs rs6265 and rs11030101), allele distribution and frequency distribution of combined genotypes and schizophrenia, and the correlation between PANSS and PSP were analyzed.
Research findings:
There was no significant difference in the average course of disease and age between the two groups (P 0.05). The average score of PSP in the experimental group was (46.04 +16.02), indicating that the patients'social cognition and social function had been impaired in different degrees. There was no significant difference in clinical classification PSP score (P 0.05); serum BDNF in experimental group was lower than that in control group, the difference was significant (P 0.05); there was no significant difference in serum BDNF concentration between the two groups (P 0.05); there was no significant difference in serum BDNF concentration between the two groups at different ages (P 0.05). There was no significant difference in serum BDNF concentration among patients with different clinical diagnosis types (P 0.05). The orthogonal correlation analysis of serum BDNF concentration, PSP score and PANSS score showed that there was a negative correlation between PSP score and PANSS score, and the difference was significant (P 0.05). Significance correlation (P 0.05); There was no significant difference in genotype and allele between the two groups (P 0.05); SNPs rs6265 and rs11030101 genotypes and allele frequencies between male and female patients in the two groups had no significant difference (P 0.05); SNPs rs6265 and rs11030101 genotypes and allele frequencies in the two groups had no significant difference (P 0.05). There was no significant difference in the frequency distribution of alleles between men and women (P 0.05); there was no significant difference in the frequency distribution of combined genotypes (SNPs rs6265 rs11030101) between the two groups (P 0.0.5); there was no significant difference in the distribution of genotypes and alleles of SNPs rs6265 and rs11030101 between the two groups (P 0.05). There was no significant difference in the genotype and allele distribution of SNPs rs6265 and rs11030101 among the patients with different clinical diagnosis types (P 0.05); there was no significant difference in the PANSS score between the patients with different genotypes of SNPs rs6265 and rs11030101 in the experimental group (P 0.05); there was no significant difference in the PSP score among the patients with different genotypes of SNPs rs6265. Significance (P 0.05), different genotypes of SNPs rs11030101 patients with the highest PSP score G/A, the lowest A/A, the difference was significant (P 0.05); experimental group of different SNPs rs6265 and rs11030101 genotype serum BDNF concentration difference was not statistically significant (P 0.05).
Conclusion:
The level of serum BDNF in schizophrenic patients was lower than that in normal subjects. Different genotypes at SNPs rs11030101 locus may have an impact on individual and social function scores of patients with schizophrenia, of which G/A type had the highest impact and A/A type had the lowest impact.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R749.3
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