穴位埋线联合东莨菪碱治疗对吗啡依赖大鼠十二指肠表达胃泌素和生长抑素的影响
发布时间：2018-12-09 17:09
【摘要】：目的:探讨穴位埋线加东莨胆碱治疗对吗啡依赖大鼠十二指肠胃泌素(Gas)、生长抑素(SS)免疫反应(IR)细胞的影响。方法:将大鼠大鼠随机分为正常组、自然戒断组(简称戒断组)、东莨菪碱治疗组(简称西药组)、穴位埋线治疗组(简称穴位埋线组)及穴位埋线加东莨菪碱治疗组(简称穴位埋线加西药组)。经腹腔注射盐酸吗啡建立吗啡依赖大鼠模型,采用不同的方法干预,观察戒断症状,分别于干预后7 d和14 d处死取材,观察十二指肠黏膜Gas和SS-IR形态特点,并比较各组十二指肠组织匀浆中Gas和SS的含量。结果:吗啡依赖大鼠戒断症状明显,其十二指肠黏膜Gas和SS-IR细胞数量明显增加(P0.05),组织匀浆中Gas和SS含量明显增加(P0.01);治疗7 d后,穴位埋线加西药组各指标明显改善,与正常组无显著差异(P0.05),治疗时间延长至14 d,西药组与穴位埋线组各项指标与正常组接近(P0.05)。结论:西药治疗与穴位埋线治疗对吗啡依赖所致大鼠的戒断症状及十二指肠分泌Gas和SS的功能均有逆转作用,两种方法结合可缩短治疗时间,治疗效果更佳。
[Abstract]:Aim: to investigate the effect of acupoint catgut embedding plus dongunachine on the immunoreactive (IR) cells of (Gas), somatostatin (SS) in duodenum of morphine dependent rats. Methods: rats were randomly divided into normal group, natural abstinence group (abstinence group) and scopolamine treatment group (western medicine group). Acupoint catgut embedding treatment group (abbreviated as acupoint embedding group) and acupoint embedding thread plus scopolamine treatment group (acupoint catgut embedding plus western medicine group). Morphine dependent rat model was established by intraperitoneal injection of morphine hydrochloride. The withdrawal symptoms were observed by different methods. The rats were killed at 7 and 14 days after intervention, and the morphologic characteristics of Gas and SS-IR in duodenal mucosa were observed. The contents of Gas and SS in duodenum homogenate of each group were compared. Results: the abstinence symptoms of morphine dependent rats were obvious, the number of Gas and SS-IR cells in duodenal mucosa were significantly increased (P0.05), and the contents of Gas and SS in tissue homogenate were significantly increased (P0.01). After 7 days of treatment, the indexes of acupoint catgut embedding plus western medicine group were improved obviously, there was no significant difference between the group and the normal group (P0.05), the treatment time was prolonged to 14 days, and the indexes of western medicine group and acupoint embedding group were close to those of the normal group (P0.05). Conclusion: Western medicine therapy and acupoint embedding therapy can reverse the withdrawal symptoms of morphine dependent rats and the function of duodenal secretion of Gas and SS. The combination of the two methods can shorten the treatment time and the therapeutic effect is better.
【作者单位】： 贵阳中医学院组织学与胚胎学教研室;贵阳中医学院形态学实验室;贵阳中医学院针灸推拿学院;澳门大学中华医药研究院;
【基金】：贵州省科技厅、贵阳中医学院联合基金项目(黔科合中药字[2011]LKZ7040号)
【分类号】：R749.64
本文编号：2369741
[Abstract]:Aim: to investigate the effect of acupoint catgut embedding plus dongunachine on the immunoreactive (IR) cells of (Gas), somatostatin (SS) in duodenum of morphine dependent rats. Methods: rats were randomly divided into normal group, natural abstinence group (abstinence group) and scopolamine treatment group (western medicine group). Acupoint catgut embedding treatment group (abbreviated as acupoint embedding group) and acupoint embedding thread plus scopolamine treatment group (acupoint catgut embedding plus western medicine group). Morphine dependent rat model was established by intraperitoneal injection of morphine hydrochloride. The withdrawal symptoms were observed by different methods. The rats were killed at 7 and 14 days after intervention, and the morphologic characteristics of Gas and SS-IR in duodenal mucosa were observed. The contents of Gas and SS in duodenum homogenate of each group were compared. Results: the abstinence symptoms of morphine dependent rats were obvious, the number of Gas and SS-IR cells in duodenal mucosa were significantly increased (P0.05), and the contents of Gas and SS in tissue homogenate were significantly increased (P0.01). After 7 days of treatment, the indexes of acupoint catgut embedding plus western medicine group were improved obviously, there was no significant difference between the group and the normal group (P0.05), the treatment time was prolonged to 14 days, and the indexes of western medicine group and acupoint embedding group were close to those of the normal group (P0.05). Conclusion: Western medicine therapy and acupoint embedding therapy can reverse the withdrawal symptoms of morphine dependent rats and the function of duodenal secretion of Gas and SS. The combination of the two methods can shorten the treatment time and the therapeutic effect is better.
【作者单位】： 贵阳中医学院组织学与胚胎学教研室;贵阳中医学院形态学实验室;贵阳中医学院针灸推拿学院;澳门大学中华医药研究院;
【基金】：贵州省科技厅、贵阳中医学院联合基金项目(黔科合中药字[2011]LKZ7040号)
【分类号】：R749.64
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