骨髓间充质干细胞输注对血管性痴呆大鼠海马细胞凋亡的影响

发布时间：2019-01-06 07:57

【摘要】：目的观察颈内动脉输注骨髓间充质干细胞(marrow stemcells,MSC)对血管性痴呆(vascular dementia,VaD)大鼠海马细胞凋亡及认知功能的影响。方法取幼年雄性清洁Wistar大鼠股骨骨髓单核细胞,贴壁培养后,取3~4代MSC诱导分化,用5-溴脱氧尿核苷(BrdU)标记。选老龄Wistar大鼠90只分为对照组、模型组、治疗组,每组30只,每组又分为2、4、8周3个时间点,每个时间点10只。制作VaD大鼠模型,治疗组于术后24h颈内动脉移植0.5ml 1.2×107/ml的BrdU标记细胞。术后2、4、8周3个时间点以穿梭箱对大鼠行为学进行检测。用TUNEL法对大鼠海马凋亡细胞进行检测。结果与对照组比较,模型组和治疗组大鼠术后2、4、8周学习记忆显著降低(P0.01);与模型组比较,治疗组大鼠术后2、4、8周学习记忆显著改善(P0.01)。治疗组大鼠术后2、4、8周海马凋亡细胞比例显著低于模型组(P0.01)。结论颈内动脉移植MSC对VaD大鼠海马细胞有明显保护作用,且显著改善VaD大鼠的学习记忆能力。
[Abstract]:Objective to investigate the effects of internal carotid artery infusion of bone marrow mesenchymal stem cells (marrow stemcells,MSC) on hippocampal apoptosis and cognitive function in vascular dementia (vascular dementia,VaD) rats. Methods Bone marrow mononuclear cells from young male clean Wistar rats were harvested and cultured with adherent cells. The differentiation was induced by 3 ~ 4 passages of MSC and then labeled with 5-bromodeoxyuridine (5-bromodeoxyuridine) (BrdU). Ninety aged Wistar rats were divided into control group, model group and treatment group with 30 rats in each group. Each group was divided into 2 groups, 4 weeks, 3 time points, each time point 10. The VaD rat model was established. In the treatment group, the 0.5ml 1.2 脳 107/ml BrdU labeled cells were transplanted into the internal carotid artery 24 hours after operation. The behavior of rats was detected by shuttle box at 3 time points at 8 weeks after operation. The apoptotic cells in hippocampus of rats were detected by TUNEL method. Results compared with the control group, the learning and memory of the model group and the treatment group decreased significantly at 8 weeks after operation (P0.01), and the learning and memory of the treatment group improved significantly at the 8th week after operation (P0.01). The percentage of apoptotic cells in hippocampus of the treatment group was significantly lower than that of the model group (P0.01). Conclusion Internal carotid artery transplantation (MSC) has obvious protective effect on hippocampal cells in VaD rats and can improve the learning and memory ability of VaD rats.
【作者单位】：解放军150中心医院重症医学科;解放军150中心医院神经内科;
【基金】：“十一五”军队科技攻关项目(NO06G076)
【分类号】：R749.13

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