磁共振波谱成像技术检测VD大鼠海马区神经元、星形胶质细胞的代谢改变及脑通汤的干预作用
发布时间：2019-04-25 11:57
【摘要】：目的通过观察血管性痴呆(VD)大鼠模型学习记忆能力的改变,以VD大鼠大脑神经血管单元为观察靶点,运用磁共振波谱技术分别检测VD病程中1、2、3、4周海马区神经元、星形胶质细胞的代谢,进而阐明脑通汤治疗血管性痴呆的相关作用机制。 方法SD大鼠60只随机分为4组：①假手术组②模型组③多奈哌齐组④脑通汤组,每组15只。经Morris水迷宫检测筛选后,后3组合格大鼠采用改良的4血管阻断法(4-VO)进行造模。造模后进行灌胃治疗,每日1次,共4周。分别于造模后1、2、3、4周运用磁共振波谱技术检测大鼠VD病程中海马区神经元、星形胶质细胞的代谢改变 结果模型组NAA/Cr比值降低,Cho/Cr比值增高,相对于假手术组来说,具有统计学意义(PO.O1～P0.05),这表明经过VD造模后使大鼠大脑海马区代谢物发生改变,即神经元活力降低以及胶质细胞过度增生。脑通汤组能显著提高大脑海马区NAA/Cr的含量、降低Cho/Cr比值,与模型组相比具有统计学意义(P0.01-P0.05),说明此方具有较好的改善神经元的受损或丢失并抑制胶质细胞过度增生的作用,对血管性痴呆的发生和发展有较好的延缓和改善的疗效,且这种作用优于盐酸多奈哌齐,是脑通汤在临床中改善VD病人学习记忆能力的干预因素和治疗机制之一。 结论改良的四血管阻断法(4-VO)制作的VD模型表现为学习记忆功能下降,该模型可用作血管性痴呆动物模型。脑通汤组具有较好的改善神经元的受损或丢失并抑制胶质细胞过度增生的作用,对血管性痴呆的发生和发展有较好的延缓和改善的疗效,且这种作用优于盐酸多奈哌齐。
[Abstract]:Objective to observe the changes of learning and memory ability in (VD) rats with vascular dementia (VD), and to detect the hippocampal neurons in the course of VD by using magnetic resonance spectroscopy (Mrs) at 1, 2, 3, 4 weeks, respectively, taking the neurovascular unit of the brain of VD rats as the target. The metabolism of astrocytes further elucidates the mechanism of Naotong decoction in the treatment of vascular dementia. Methods Sixty SD rats were randomly divided into 4 groups: 1 sham operation group 2 model group 3 Donepezil group 4 Naotong decoction group with 15 rats in each group. After screening by Morris water maze test, the model was made by modified 4-vessel occlusion (4-VO) method in the latter 3 groups. After the model was made, the rats were treated by gastric perfusion once a day for a total of 4 weeks. The metabolic changes of hippocampal neurons and astrocytes in rats during the course of VD were detected by magnetic resonance spectroscopy at 1, 2, 3, 4 weeks after the establishment of the model. The results showed that the ratio of NAA/Cr to Cho/Cr in the model group was lower and the ratio of astrocytes was higher than that in the model group. Compared with the sham-operated group, it was statistically significant (PO.O1~P0.05), which indicated that after VD modeling, the metabolites in the hippocampus of rats were changed, that is, the activity of neurons was decreased and the glial cells were over-proliferated. Naotong decoction group can significantly increase the content of NAA/Cr in the hippocampus and decrease the ratio of Cho/Cr, which has statistical significance (P0.01-P0.05) compared with the model group. It shows that this prescription has a better effect on improving the damage or loss of neurons and inhibiting the excessive proliferation of glial cells, and has a better effect on delaying and improving the occurrence and development of vascular dementia, and this effect is better than that of Donepezil Hydrochloride. It is one of the intervention factors and therapeutic mechanism of Naotong decoction to improve the learning and memory ability of VD patients. Conclusion the improved four-vessel occlusion (4-VO)-induced VD model is characterized by learning and memory impairment, and can be used as an animal model of vascular dementia. Naotong decoction group had a better effect on improving the damage or loss of neurons and inhibiting the excessive proliferation of glial cells, and had a better effect on delaying and improving the occurrence and development of vascular dementia, and this effect was better than that of Donepezil Hydrochloride.
【学位授予单位】：贵阳中医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R445.2;R749.13
本文编号：2465066
[Abstract]:Objective to observe the changes of learning and memory ability in (VD) rats with vascular dementia (VD), and to detect the hippocampal neurons in the course of VD by using magnetic resonance spectroscopy (Mrs) at 1, 2, 3, 4 weeks, respectively, taking the neurovascular unit of the brain of VD rats as the target. The metabolism of astrocytes further elucidates the mechanism of Naotong decoction in the treatment of vascular dementia. Methods Sixty SD rats were randomly divided into 4 groups: 1 sham operation group 2 model group 3 Donepezil group 4 Naotong decoction group with 15 rats in each group. After screening by Morris water maze test, the model was made by modified 4-vessel occlusion (4-VO) method in the latter 3 groups. After the model was made, the rats were treated by gastric perfusion once a day for a total of 4 weeks. The metabolic changes of hippocampal neurons and astrocytes in rats during the course of VD were detected by magnetic resonance spectroscopy at 1, 2, 3, 4 weeks after the establishment of the model. The results showed that the ratio of NAA/Cr to Cho/Cr in the model group was lower and the ratio of astrocytes was higher than that in the model group. Compared with the sham-operated group, it was statistically significant (PO.O1~P0.05), which indicated that after VD modeling, the metabolites in the hippocampus of rats were changed, that is, the activity of neurons was decreased and the glial cells were over-proliferated. Naotong decoction group can significantly increase the content of NAA/Cr in the hippocampus and decrease the ratio of Cho/Cr, which has statistical significance (P0.01-P0.05) compared with the model group. It shows that this prescription has a better effect on improving the damage or loss of neurons and inhibiting the excessive proliferation of glial cells, and has a better effect on delaying and improving the occurrence and development of vascular dementia, and this effect is better than that of Donepezil Hydrochloride. It is one of the intervention factors and therapeutic mechanism of Naotong decoction to improve the learning and memory ability of VD patients. Conclusion the improved four-vessel occlusion (4-VO)-induced VD model is characterized by learning and memory impairment, and can be used as an animal model of vascular dementia. Naotong decoction group had a better effect on improving the damage or loss of neurons and inhibiting the excessive proliferation of glial cells, and had a better effect on delaying and improving the occurrence and development of vascular dementia, and this effect was better than that of Donepezil Hydrochloride.
【学位授予单位】：贵阳中医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R445.2;R749.13
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