癸酸对AD小鼠的影响作用研究
发布时间：2019-05-22 02:51
【摘要】：目的:建立AD(Alzheimer disease)小鼠动物模型,研究癸酸(DA)对其影响作用。方法:将50只雄性昆明种小鼠随机分为正常组,模型组,低剂量给药组(DA 50mg/kg),中剂量给药组(DA 100mg/kg),和高剂量给药组(DA 200mg/kg)共5个实验组。正常组小鼠颈背部注射生理盐水,模型组和癸酸给药组颈背部皮下均注射D-半乳糖溶液。低剂量给药组,中剂量给药组,和高剂量给药组小鼠皮下注射癸酸。8周后观察小鼠行为学变化,取小鼠脑组织观察脑神经元病理学变化,检测脑组织中PPARγ、NFκB蛋白含量以及检测血清中TNF-α、i NOS因子的水平。结果:水迷宫测试中癸酸各剂量给药组小鼠学习记忆能力提高;病理学结果显示癸酸给药后神经细胞损伤程度减弱,均低于模型组;小鼠脑组织中PPARγ,NFκB,以及血清中TNF-α、i NOS的含量均低于模型组。以高剂量给药组最为显著。结论:癸酸可以改善AD模型小鼠的一定程度的学习记忆能力,减轻神经元损伤程度,降低AD小鼠某些炎性成分表达,其作用机制可能通过激动PPAR-γ表达减少NFκB、TNF-α、i NOS的生成有关。
[Abstract]:Objective: to establish an animal model of AD (Alzheimer disease) mice and study the effect of decanoic acid (DA) on it. Methods: fifty male Kunming mice were randomly divided into five experimental groups: normal group, model group, low dose administration group (DA 50mg/kg), middle dose administration group (DA 100mg/kg) and high dose administration group (DA 200mg/kg). Normal saline was injected into the neck and back of the normal group, and D-galactose solution was injected subcutaneously into the neck and back of the model group and decanoic acid group. The mice in the low dose administration group, the middle dose administration group and the high dose administration group were subcutaneously injected with decanoic acid. After 8 weeks, the behavioral changes of the mice were observed, the pathological changes of brain neurons were observed in the brain tissue of the mice, and the PPAR 纬 in the brain tissue was detected. The content of NF kappa B protein and the level of TNF- 伪, i NOS factor in serum were detected. Results: in the water maze test, the learning and memory ability of the mice in each dose of decanoic acid group was improved, and the pathological results showed that the degree of nerve cell injury decreased after decanoic acid administration, which was lower than that in the model group. The contents of PPAR 纬, NF kappa B and TNF- 伪, i NOS in brain tissue of mice were lower than those in model group. The high dose group was the most significant. Conclusion: decanoic acid can improve the learning and memory ability of AD model mice, reduce the degree of neuronal injury and decrease the expression of some inflammatory components in AD mice. The mechanism of decanoic acid may decrease NF kappa B, TNF伪 by activating the expression of PPAR- 纬. The generation of I NOS is related.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749.16
[Abstract]:Objective: to establish an animal model of AD (Alzheimer disease) mice and study the effect of decanoic acid (DA) on it. Methods: fifty male Kunming mice were randomly divided into five experimental groups: normal group, model group, low dose administration group (DA 50mg/kg), middle dose administration group (DA 100mg/kg) and high dose administration group (DA 200mg/kg). Normal saline was injected into the neck and back of the normal group, and D-galactose solution was injected subcutaneously into the neck and back of the model group and decanoic acid group. The mice in the low dose administration group, the middle dose administration group and the high dose administration group were subcutaneously injected with decanoic acid. After 8 weeks, the behavioral changes of the mice were observed, the pathological changes of brain neurons were observed in the brain tissue of the mice, and the PPAR 纬 in the brain tissue was detected. The content of NF kappa B protein and the level of TNF- 伪, i NOS factor in serum were detected. Results: in the water maze test, the learning and memory ability of the mice in each dose of decanoic acid group was improved, and the pathological results showed that the degree of nerve cell injury decreased after decanoic acid administration, which was lower than that in the model group. The contents of PPAR 纬, NF kappa B and TNF- 伪, i NOS in brain tissue of mice were lower than those in model group. The high dose group was the most significant. Conclusion: decanoic acid can improve the learning and memory ability of AD model mice, reduce the degree of neuronal injury and decrease the expression of some inflammatory components in AD mice. The mechanism of decanoic acid may decrease NF kappa B, TNF伪 by activating the expression of PPAR- 纬. The generation of I NOS is related.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749.16
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