TLR2和TLR4在人慢性根尖周病组织肥大细胞上的表达

发布时间：2018-01-12 07:07

  本文关键词：TLR2和TLR4在人慢性根尖周病组织肥大细胞上的表达　出处：《中国病理生理杂志》2016年03期 　论文类型：期刊论文

  更多相关文章： 慢性根尖周病 肥大细胞 Toll样受体 Toll样受体

【摘要】：目的:观察人不同类型慢性根尖周病组织中肥大细胞(mast cells,MCs)上Toll样受体2(Toll-like receptor 2,TLR2)和TLR4的表达情况,探讨TLR2-类胰蛋白酶(tryptase)和TLR4-tryptase双阳性MCs在慢性根尖周疾病发病机制中的作用。方法:将60例自愿参与本研究的受试者按根尖周病分类标准分为3组:(1)正常对照组;(2)根尖周肉芽肿组;(3)根尖周囊肿组。将根尖周组织标本置于4%甲醛固定液中浸泡48 h以上,制作连续组织切片。HE染色,光学显微镜下观察各组根尖周标本的组织学变化;免疫荧光双染色后于荧光显微镜下观察TLR2-tryptase和TLR4-tryptase双阳性MCs在根尖周组织中的分布情况。结果:根尖周病变组TLR2-tryptase和TLR4-tryptase双阳性MCs比正常牙周膜组明显增多(P0.01);与根尖肉芽肿组相比,根尖囊肿组TLR2-tryptase及TLR4-tryptase双阳性MCs数目明显增高(P0.01)。结论:TLR2及TLR4在慢性根尖周疾病组织中MCs上表达增加,提示TLR2-tryptase及TLR4-tryptase双阳性MCs可能参与慢性根尖周病发病过程的免疫调节。
[Abstract]:Objective: to observe the Toll like receptor 2 Toll-like receptor 2 on mast cells of different types of chronic periapical disease. TLR2) and TLR4 expression. Study on TLR2-Tryptase. Methods: 60 subjects who volunteered to participate in this study were divided into 3 groups according to the classification criteria of periapical disease. 1) normal control group; 2) periapical granuloma group; 3) periapical cyst group. The periapical tissue samples were soaked in 4% formaldehyde fixative solution for more than 48 hours, and the tissue sections were stained with HE. The histological changes of periapical specimens were observed under optical microscope. The distribution of TLR2-tryptase and TLR4-tryptase double positive MCs in periapical tissues was observed under fluorescence microscope after double immunofluorescence staining. The number of TLR2-tryptase and TLR4-tryptase double positive MCs in periapical lesion group was significantly higher than that in normal periodontal ligament group. P0.01; Compared with the apical granuloma group. The number of TLR2-tryptase and TLR4-tryptase double positive MCs in the apical cyst group was significantly higher than that in the control group (P 0.01). Conclusion the expression of TLR2 and TLR4 in the tissues of chronic periapical diseases is increased. The results suggest that TLR2-tryptase and TLR4-tryptase double positive MCs may be involved in the immunomodulation of chronic periapical disease.
【作者单位】： 暨南大学医学院口腔医学系;
【基金】：广东省社会发展领域科技计划(No.2013B021800043;No.2014A020212212)
【分类号】：R781.34
【正文快照】： 慢性根尖周疾病是口腔常见疾病之一,主要由来源于感染根管的细菌及其代谢产物扩散至根尖周组织所致,表现为根尖周组织的炎症与骨吸收。传统认为,肥大细胞(mast cells,MCs)是过敏性炎症反应的主要效应细胞,近年的研究发现,MCs作为效应和调节性免疫细胞在固有免疫的建立和适应性，

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