RANKL和OPG对不同骨移植材料行位点保存中骨构建的作用

发布时间：2018-01-13 01:02

  本文关键词：RANKL和OPG对不同骨移植材料行位点保存中骨构建的作用　出处：《新疆医科大学》2015年硕士论文　论文类型：学位论文

  更多相关文章： 拔牙位点保存 骨移植 骨保护因子 核因子kB受体活化因子配体 破骨细胞

【摘要】：目的:通过观察破骨细胞核因子kB受体活化因子配体和骨保护因子在使用不同骨移植材料行拔牙位点保存时骨构建过程中的表达变化,探讨破骨细胞对不同骨移植材料植入拔牙窝内在引导骨再生中的作用与意义。材料及方法:选取六只12月龄雄性beagle犬,微创拔除其上下颌双侧第一侧切牙,将四个位点随机做不同处理,分组为:A组:填入富血小板纤维蛋白(Platelet-rich fibrin,PRF),B组:填入骨诱导活性材料(osteoinduction active material,OAM),C组:填入羟基磷灰石生物陶瓷(coralline hydroxyapatite,CHA),D组:空置(对照)。六只犬随机分别于术后4周、术后12周各处死3只,制作牙槽骨标本。采用免疫组化技术检测24块拔牙位点保存制作的犬牙槽骨标本中RANKL与OPG的表达变化。结果:4周处死标本骨组织中OPG的表达A组依次高于B组,C组和D组(P0.05),RANKL的表达D组依次高于C组,B组和A组(P0.05),12周处死标本骨组织中OPG的表达D组依次高于C组,B组和A组(P0.05)。RANKL的表达C组依次高于B组,A组和D组(P0.05)。结论:骨移植材料在骨改建过程中通过促进成骨细胞分化成熟而刺激OPG表达升高,抑制破骨细胞,从而加强成骨作用,有效提高新骨形成量,提示骨移植材料通过调控RANKL和OPG在拔牙窝新骨形成的过程中发挥着重要的作用。
[Abstract]:Objective: to observe the expression changes of osteoclast nuclear factor kB receptor activating factor ligand and bone protection factor during bone construction during the preservation of extraction sites using different bone graft materials. To explore the role and significance of osteoclasts in guiding bone regeneration by implantation of different bone graft materials into extraction fossa. Materials and methods: six 12-month-old male beagle dogs were selected. The maxillary and mandibular first incisors were extracted by minimally invasive extraction, and the four sites were randomly divided into two groups: group A: Platelet-rich fibrin filled with platelet-rich fibrin. Group B: osteoinductive active material (OAM). Group C: hydroxyapatite bioceramics: group D: empty (control group): 6 dogs were randomly assigned 4 weeks after operation. Three rats died 12 weeks after operation. The expression of RANKL and OPG in 24 canine alveolar bone specimens preserved at extraction sites were detected by immunohistochemical technique. The expression of OPG in bone tissue of group A was higher than that of group B at 4 weeks. The expression of OPG in group D was higher than that in group C (P 0.05) and group A (P 0.05) at 12 weeks. The expression of OPG in group D was higher than that in group C (P 0.05). The expression of P0.05, RANKL in group B and group A was higher than that in group B, respectively. Conclusion: during bone remodeling, bone graft materials stimulate the expression of OPG and inhibit osteoclasts in the process of bone remodeling by promoting the differentiation and maturation of osteoblasts, thus enhancing the osteogenic effect. It is suggested that bone graft material plays an important role in the formation of new bone in the extraction of dental fossa by regulating RANKL and OPG.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R782.11
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本文编号：1416746