富血小板纤维蛋白和浓缩生长因子对施万细胞影响的比较

发布时间：2018-01-17 03:05

  本文关键词：富血小板纤维蛋白和浓缩生长因子对施万细胞影响的比较　出处：《中国医科大学学报》2016年12期 　论文类型：期刊论文

  更多相关文章： 富血小板纤维蛋白 浓缩生长因子 施万细胞 神经生长因子 细胞周期

【摘要】：目的以施万细胞作为周围神经模型,比较富血小板纤维蛋白(PRF)与浓缩生长因子(CGF)对其的影响。方法随机选取18~55岁身体健康志愿者10人,无菌条件下采集静脉血10 m L,制备PRF和CGF。将细胞随机分为对照组、PRF组和CGF组。倒置相差显微镜下观察细胞形态;MTT比色法测定细胞增殖能力;酶联免疫吸附法检测细胞上清液中神经生长因子的分泌量;流式细胞仪检测细胞周期。结果倒置相差显微镜下观察各组细胞形态无明显差异;MTT结果显示PRF组和CGF组与对照组相比光密度值明显升高(P0.05),细胞上清液中神经生长因子分泌量均明显升高(P0.05),处于S+G2M期的细胞数明显增多(P0.01),而PRF组与CGF组相比各项指标的差异无统计学意义。结论 PRF和CGF均可以促进施万细胞增殖并增加神经生长因子的分泌量,但就改善细胞增殖能力、促进神经生长因子分泌来看,二者无明显差异。
[Abstract]:Objective to use Schwann cells as peripheral nerve model. The effects of platelet rich fibrin (PRF) and concentrated growth factor (CGF) on it were compared. Methods 10 healthy volunteers aged from 18 to 55 years were randomly selected. PRF and CGF were prepared by collecting 10 mL venous blood under aseptic condition. The cells were randomly divided into two groups: control group and CGF group. The morphology of the cells was observed under inverted phase contrast microscope. Cell proliferation was measured by MTT colorimetry. Enzyme linked immunosorbent assay (Elisa) was used to detect the secretion of nerve growth factor (NGF) in the supernatant. Results there was no significant difference in cell morphology under inverted phase contrast microscope. MTT results showed that the optical density of PRF group and CGF group was significantly higher than that of control group, and the secretion of nerve growth factor in cell supernatant was significantly higher than that of control group (P 0.05). The number of cells in S G 2m phase increased significantly (P 0.01). Conclusion both PRF and CGF can promote the proliferation of Schwann cells and increase the secretion of nerve growth factor. However, there was no significant difference between the two groups in terms of improving cell proliferation and promoting the secretion of nerve growth factor (NGF).
【作者单位】： 锦州医科大学附属第二医院口腔种植中心;中国医科大学附属盛京医院集团抚顺医院口腔科;
【基金】：辽宁省科技厅优秀人才培育项目(2014022025)
【分类号】：R783.6
【正文快照】： 113000)口腔种植技术正处于飞速发展与日益完善的黄金时期,越来越多的牙齿缺失患者放弃了传统镶牙而选择口腔种植修复,后者无论是在美观还是实用性上与传统镶牙相比都有较大优势。牙周膜组织中含有大量鲁菲尼小体,它们是天然牙的机械感受器,对于牙齿缺失的患者来说,牙周膜组织，

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