高压氧疗法对大鼠心理应激相关牙周炎治疗作用及低氧诱导因子1α表达的研究

发布时间：2018-01-19 03:20

本文关键词： 牙周炎心理应激高压氧低氧诱导因子-α　出处：《中国病理生理杂志》2016年02期 　论文类型：期刊论文

【摘要】：目的:探讨高压氧(hyperbaric oxygen,HBO)治疗对心理应激相关牙周炎牙周组织低氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)表达的影响。方法:清洁级4周龄雄性Wistar大鼠120只,随机分为4组:正常对照组;单纯应激组;牙周炎组:用浸有牙龈卟啉单胞菌株的丝线结扎左侧上颌第二磨牙牙颈部,复制实验性牙周炎模型;牙周炎+应激组。于实验后第9周停止应激刺激,对除正常对照组外其它各组大鼠每组选取6只进行HBO治疗。于实验后2、4和8周末分批处死未治疗动物,每组处死6只;于实验后10周末处死HBO治疗及未治疗动物。处死前测量术区的牙周附着情况,制作牙体牙周联合切片,观察牙周的组织学改变。免疫组化法检测各组大鼠牙周组织HIF-1α的表达水平,计算平均阳性细胞率。结果:大体观察见正常对照组及单纯应激组牙周附着位置正常;牙周炎组出现牙龈萎缩,附着丧失明显;牙周炎+应激组附着丧失明显,根分叉暴露,HBO治疗后,牙龈水肿减轻,牙周袋变浅;组织学观察见牙周炎+应激组牙周组织破坏程度在各时点均重于牙周炎组;经HBO治疗后,牙周组织炎症程度减轻,炎症细胞浸润减少;牙龈指数(gingival index,GI)及牙周附着丧失(attachment loss,AL)检查见单纯应激组与正常对照组在各时点均无显著差异;在第4、8周时牙周炎+应激组GI、AL明显高于牙周炎组(P0.01);HBO治疗结束后牙周炎组及牙周炎+应激组GI、AL明显低于未治疗组(P0.05);单纯应激组与正常对照组HIF-1α免疫组化平均阳性细胞率在各时点均无显著差异;牙周炎+应激组HIF-1α平均阳性细胞率在第4、8周时均明显高于牙周炎组(P0.01);HBO治疗结束后牙周炎组及牙周炎+应激组HIF-1α平均阳性细胞率较未治疗组降低(P0.01)。结论:心理应激可加重牙周组织缺氧程度从而加重牙周炎症状,HBO通过增加牙周组织的氧供应量对大鼠实验性牙周炎及心理应激相关牙周炎有显著的治疗效果。
[Abstract]:Objective: to study hyperbaric oxygen with hyperbaric oxygen. HBO) treatment on hypoxia-inducible factor-1 伪 and hypoxia-inducible factor-1 伪 in periodontal tissue of periodontitis associated with psychological stress. Methods: 120 male Wistar rats of clean grade 4 weeks old were randomly divided into 4 groups: normal control group; Simple stress group; In the periodontitis group, experimental periodontitis model was established by ligating the neck of the left maxillary second molar with filaments impregnated with porphyromonas gingivalis. Periodontitis stress group. At the 9th week after the experiment, the stress stimulation was stopped, and 6 rats in each group except the normal control group were treated with HBO. 2. At the end of 4 and 8 weeks, the untreated animals were killed in batches and 6 animals were killed in each group. HBO and untreated animals were killed at the end of 10 weeks after the experiment. Periodontal attachment was measured before death and periodontal joint sections were made. Immunohistochemical method was used to detect the expression of HIF-1 伪 in periodontal tissues of rats. Results: the normal periodontal attachment was observed in normal control group and simple stress group. In periodontitis group, gingival atrophy and loss of attachment were observed. After HBO treatment, gingival edema was alleviated and the periodontal bag shallowed in the stress group of periodontitis. Histological observation showed that the degree of periodontal tissue destruction in periodontitis stress group was more serious than that in periodontitis group at each time point. After HBO treatment, the degree of periodontal inflammation was reduced and the infiltration of inflammatory cells was decreased. Gingival index (GI) and periodontal attachment attachment (loss). There was no significant difference between the simple stress group and the normal control group at all time points. At the 4th week, GIIA AL in periodontitis stress group was significantly higher than that in periodontitis group (P 0.01). After HBO treatment, the levels of GI AL in periodontitis group and periodontitis stress group were significantly lower than those in untreated group (P 0.05). There was no significant difference in the average positive rate of HIF-1 伪 immunohistochemistry between the simple stress group and the normal control group at each time point. The average positive rate of HIF-1 伪 in periodontitis stress group was significantly higher than that in periodontitis group at the 8th week. The average positive rate of HIF-1 伪 in periodontitis group and periodontitis stress group after HBO treatment was lower than that in untreated group (P 0.01). Conclusion: psychological stress can aggravate the degree of hypoxia in periodontal tissue and aggravate the symptoms of periodontitis. HBO has a significant therapeutic effect on experimental periodontitis and psychological stress-related periodontitis in rats by increasing oxygen supply in periodontal tissue.
【作者单位】：暨南大学医学院口腔医学系;
【基金】：广东省社会发展领域科技计划项资助项目(No.2013B021800043;No.2014A020212212)
【分类号】：R781.42
【正文快照】： 牙周炎是侵犯牙龈和牙周组织的一种慢性炎症性疾病,主要特征为牙周袋的形成、袋壁的炎症和牙槽骨吸收,是导致成年人牙齿丧失的主要原因[1]。研究表明,微生物是牙周病的启动因子,心理应激与炎症性疾病如类风湿性关节炎和牙周病之间有关联[2],是牙周病潜在的重要的风险因子[3]。

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