microRNA-21在口腔鳞状细胞癌中的表达与预后关系

发布时间：2018-01-19 04:13

本文关键词： 口腔鳞状细胞癌微 RNA- 表达预后　出处：《口腔医学研究》2015年09期 　论文类型：期刊论文

【摘要】：目的:探讨microRNA-21在口腔鳞状细胞癌中的表达情况,以及与预后关系。方法:选取98例口腔鳞状细胞癌患者组织及正常黏膜组织标本,同时,选取50例非肿瘤患者黏膜组织,分别提取标本中总RNA,利用RT-PCR检测microRNA-21在口腔鳞状细胞癌及正常黏膜组织中相对表达量,探讨其与临床病理特征的关系,利用Kaplan-Meier法分析其与预后的关系。结果:microRNA-21在口腔鳞状细胞癌组织中相对表达量(2.37±1.01),显著高于正常黏膜组织中的(0.97±0.08)和非肿瘤患者黏膜组织中的(0.94±0.07),差异具有统计学意义(P0.05),;microRNA-21表达与患者性别、年龄、肿瘤位置、肿瘤大小和TNM分期无关,而与分化程度和是否发生淋巴结转移有关(P0.05);将microRNA-21相对表达量的中位值2.41为界,将所有患者分为高表达组和低表达组,高表达组患者中位生存期38个月,低表达组患者中位生存期44个月,差异具有统计学意义(P0.05)。结论:microRNA-21在口腔鳞状细胞癌中呈高表达,且与分化程度和淋巴结转移有关,并可影响患者预后。
[Abstract]:Objective: to investigate the expression of microRNA-21 in oral squamous cell carcinoma (OSCC) and its relationship with prognosis. At the same time, the total RNA was extracted from the mucosal tissues of 50 cases of non-tumor patients. RT-PCR was used to detect the relative expression of microRNA-21 in oral squamous cell carcinoma (OSCC) and normal mucosa, and to explore the relationship between microRNA-21 and clinicopathological features. Kaplan-Meier method was used to analyze the relationship between microRNA-21 expression and prognosis. Results the relative expression of 10% microRNA-21 was 2.37 卤1.01 in oral squamous cell carcinoma (OSCC). It was significantly higher than that in normal mucosa (0.97 卤0.08) and non-tumor patients (0.94 卤0.07). The difference was statistically significant (P 0.05). The expression of microRNA-21 was not related to sex, age, tumor location, tumor size and TNM stage, but related to the degree of differentiation and lymph node metastasis. The median value of relative expression of microRNA-21 was 2.41. All patients were divided into high expression group and low expression group. The median survival time of high expression group was 38 months. In the low expression group, the median survival time was 44 months, and the difference was statistically significant (P 0.05). Conclusion the microRNA-21 is highly expressed in oral squamous cell carcinoma. And related to the degree of differentiation and lymph node metastasis, and can affect the prognosis of patients.
【作者单位】：焦煤中央医院口腔科;
【分类号】：R739.85
【正文快照】： 口腔鳞状细胞癌是人类常见的恶性肿瘤,约占口腔颌面部恶性肿瘤的80%以上,好发于40~60岁人群,易发生区域淋巴结转移,晚期可出现远处转移[1],近年来虽然诊疗手段得到很大改进,但患者5 年生存率尚不理想[2]。有研究指出[3],口腔鳞状细胞癌患者预后与诊疗时间密切相关,越早诊断并

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