乳铁蛋白下调脂多糖激发的人牙周膜细胞Toll样受体4表达的研究

发布时间：2018-02-01 09:48

本文关键词： 乳铁蛋白脂多糖人牙周膜细胞 Toll样受体　出处：《华西口腔医学杂志》2014年02期 　论文类型：期刊论文

【摘要】：目的观察乳铁蛋白(LF)对经脂多糖(LPS)刺激的人牙周膜细胞(hPDLCs)表达Toll样受体4(TLR4)的影响。方法采用组织块酶消化法培养hPDLCs,鉴定后取第4代细胞,分成空白对照组、LPS组、LPS+LF组。空白对照组不加任何刺激,LPS组加入0.1μg·mL-1 LPS;LPS+LF组在加入0.1μg·mL-1 LPS 2 h后,加入10μg·mL-1 LF。以加入LF时开始计算时间,4 h后采用实时定量聚合酶链反应(RT-PCR)法检测hPDLCs中TLR4 mRNA的表达,24 h后采用细胞免疫荧光染色法观察TLR4蛋白的表达。结果 RT-PCR检测显示:LPS+LF组TLR4 mRNA表达较LPS组明显降低(P0.05),与空白对照组无明显差异(P0.05)。细胞免疫荧光染色法显示:LPS+LF组TLR4蛋白的表达强度较LPS组减弱(P0.05),与空白对照组无明显差别(P0.05)。结论 LF可以下调LPS激发的hPDLCs中TLR4的表达,在牙周炎症TLR4信号通路的调控过程中有一定的作用。
[Abstract]:Objective to observe the effect of lactoferrin (LF) on the expression of Toll like receptor (TLR4) in human periodontal ligament cells stimulated by lipopolysaccharide (LPS). Methods hPDLCs was cultured by tissue mass enzyme digestion. After identification, the cells of the 4th passage were divided into two groups: LPS-LPs group and LPS-LF group, the control group added 0.1 渭 g 路LPS0.1 渭 g 路LPS-1 without any stimulation. In LPS LF group, 0. 1 渭 g 路mL-1 LPS was added for 2 h, then 10 渭 g 路mL-1 LF was added. The time was calculated when LF was added. After 4 h, the expression of TLR4 mRNA in hPDLCs was detected by real-time quantitative polymerase chain reaction (RT PCR). After 24 h, the expression of TLR4 protein was observed by cell immunofluorescence staining. Results the expression of TLR4 mRNA in TLR4 LF group was significantly lower than that in LPS group (P < 0.05). P0.05). There was no significant difference between the control group and the control group. Cell immunofluorescence staining showed that the expression of TLR4 protein in the LPS LF group was lower than that in the LPS group (P 0.05). Conclusion LF can down-regulate the expression of TLR4 in hPDLCs stimulated by LPS. It plays an important role in the regulation of TLR4 signal pathway in periodontitis.
【作者单位】：南方医科大学南方医院口腔科;南方医科大学口腔医学院;
【基金】：广东省科技计划基金资助项目(2010B060900053)
【分类号】：R781.4
【正文快照】： Toll样受体家族(Toll like receptors,TLRs)是一种重要的模式识别受体,可识别保守的病原体相关的分子位点激活先天性免疫系统产生炎症细胞因子,进而启动后天性免疫系统。Toll样受体4(Tolllike receptor 4,TLR4)是第一个被发现的哺乳动物的Toll样受体,可以通过经典的病原相关分

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