平阳霉素联合透明质酸钠对人淋巴管畸形内皮细胞的作用

发布时间：2018-02-03 04:41

本文关键词： 平阳霉素透明质酸钠淋巴管畸形内皮细胞　出处：《山东大学学报(医学版)》2015年01期 　论文类型：期刊论文

【摘要】：目的探讨平阳霉素(PYM)联合透明质酸钠(HA)对体外培养的人淋巴管畸形内皮细胞(HLMECs)的作用。方法对新鲜婴幼儿淋巴管畸形组织进行HLMECs原代培养。利用淋巴管内皮特异性标志物LYVE1和PROX1染色,将不同浓度(1、10、100、500μg/m L)的PYM和(50、100、300、500μg/m L)HA分别作用于HLMECs 12、24、48 h,观察对HLMECs增殖的影响并筛选其最适有效浓度。然后用该有效浓度的PYM和HA共同作用于HLMECs,在第24小时时,MTT法检测细胞生长情况,TUNEL法检测细胞凋亡情况。结果 1淋巴管畸形组织和HLMECs表达LYVE1和PROX1;2PYM的最适浓度为10μg/m L,HA的最适浓度为100μg/m L;3PYM联合HA在抑制细胞增殖和促进细胞凋亡方面比单纯的PYM作用强。结论 PYM联合HA有望在临床上用于治疗淋巴管畸形。
[Abstract]:Objective to investigate the effects of pingyangmycin (Pym) combined with sodium hyaluronate (HA) on cultured human lymphatic malformed endothelial cells (HL-MECs) in vitro. Methods the tissue of fresh infant lymphatic malformation was cultured in primary culture with HLMECs. LYVE1 and PROX1 staining were used as the specific markers of lymphatic endothelium. HLMECs 12 was treated with PYM and 500 渭 g / mL hyaluronic acid at different concentrations of 10 ~ 10 ~ 100 ~ 100 渭 g / m ~ (-1) and 500 渭 g / m ~ (-1 / mL) of #number0# 渭 g / m 路L ~ (-1) 路L ~ (-1), respectively. The effects of PYM and HA on the proliferation of HLMECs were observed and the optimal effective concentration was screened. Then the PYM and HA were used to act on HLMECs at 24 h. MTT method was used to detect cell growth and Tunel method was used to detect apoptosis. Results 1 LYVE1 and PROX1 were expressed in lymphatic malformation tissues and HLMECs. 2the optimum concentration of PYM is 10 渭 g / mL, the optimum concentration of PYM is 100 渭 g / mL; 3PYM combined with HA has stronger effect on inhibiting cell proliferation and promoting apoptosis than PYM alone. Conclusion PYM combined with HA can be used to treat lymphatic malformation in clinic.
【作者单位】：济南市第三人民医院口腔科;山东大学口腔医院口腔颌面外科;山东大学齐鲁医院口腔科;
【基金】：国家自然科学基金(81172572) 山东省自然科学基金(ZR2010HM008) 济南市科技发展计划[2011]34号
【分类号】：R782
【正文快照】： 淋巴管畸形是一种先天性的发育畸形,其虽为良性病变,但当侵犯周围重要组织器官时可导致面容破损、呼吸困难、吞咽困难等,如不及时处理,严重时可危及患者生命[1]。目前,淋巴管畸形的治疗主要采用硬化剂注射治疗,其中平阳霉素(Pingyang-mycin,PYM)局部注射硬化治疗取得较满意的

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