间质液压与涎腺腺样囊性癌恶性表型的关系及相关分子机制

发布时间：2018-02-03 10:14

本文关键词： 间质液压腺样囊性癌细胞培养压力　出处：《华西口腔医学杂志》2014年02期 　论文类型：期刊论文

【摘要】：目的探讨腺样囊性癌(ACC)细胞受力后的基因表达及调控物质变化特点,从而阐明间质液压促进ACC细胞恶性表型获得的分子基础及其作用的分子机制。方法加压(103.74 kPa)培养ACC2细胞,根据加压时间不同将实验组分为3 h组、6 h组、12 h组、24 h组。以未加压培养的ACC2为阴性对照,未加压培养的的ACCM为阳性对照。采用免疫组织化学法半定量检测各组表皮生长因子受体(EGFR)的表达,逆转录聚合酶链反应法检测基质金属蛋白酶(MMP)9和EGFR mRNA表达,Western blot法检测MMP9、EGFR、磷酸化表皮生长因子受体(P-EGFR)、角质形成细胞生长因子(KGF)、磷酸化细胞外信号调节激酶(P-ERK)蛋白表达。结果随着加压时间的延长,ACC2细胞内EGFR、P-EGFR、MMP-9、KGF、P-ERK的表达逐渐增加,整体呈时间正相关,且均高于阴性对照组。结论在力学刺激下,ACC2细胞中黏附、转移相关分子的mRNA及蛋白表达水平均升高。
[Abstract]:Objective to investigate the changes of gene expression and regulatory substances in adenoid cystic carcinoma (ACC) cells after being subjected to stress. Therefore, the molecular basis and the molecular mechanism of mesenchymal fluid stimulation for the malignant phenotype of ACC cells were elucidated. Methods ACC2 cells were cultured under pressure of 103.74 KPA. According to the time of compression, the experimental group was divided into 3 h group, 6 h group, 12 h group and 24 h group, with unpressurized ACC2 as negative control. The expression of epidermal growth factor receptor (EGFR) in each group was semi-quantitatively detected by immunohistochemical method. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of matrix metalloproteinase-9 (MMP9) and EGFR mRNA. Phosphorylated epidermal growth factor receptor (P-EGFR), keratinocyte growth factor (KGF). Results the expression of phosphorylated signal regulated kinase (P-ERK) protein in ACC2 cells was increased with the increase of pressure time. The expression of P-ERK increased gradually, and the whole expression was positively correlated with time, which was higher than that of negative control group. Conclusion the adhesion of ACC2 cells was induced by mechanical stimulation. The expression levels of mRNA and protein in metastasis related molecules were increased.
【作者单位】：口腔疾病研究国家重点实验室华西口腔医院头颈肿瘤外科(四川大学);
【基金】：教育部高等学校博士学科点专项科研基金资助项目(20-090181110082) 国家自然科学基金资助项目(30973345,81172578)
【分类号】：R739.87
【正文快照】： 涎腺腺样囊性癌(adenoid cystic carcinoma,ACC)是来自涎腺的上皮源性肿瘤,占涎腺恶性肿瘤的21.9%~24%。腺样囊性癌具有特殊的生物学行为,极易复发和远处转移,早期侵犯神经,并沿神经播散,而经区域淋巴道转移十分罕见[1]。ACC远处转移最常见的部位是肺、肝以及骨等。因此,ACC

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