高浓度葡萄糖对人牙周膜成纤维细胞成骨分化能力的影响

发布时间：2018-02-03 19:34

本文关键词： 葡萄糖牙周膜成纤维细胞成骨诱导　出处：《国际口腔医学杂志》2015年04期 　论文类型：期刊论文

【摘要】：目的研究高葡萄糖浓度刺激下牙周膜成纤维细胞(PDLC)的增殖能力和成骨分化能力。方法体外组织块法培养非糖尿病患者PDLC,分别用0 mg·L-1(C组)、1 100 mg·L-1(L组)、4 500 mg·L-1(H组)浓度葡萄糖刺激PDLC,噻唑蓝(MTT)法检测PDLC增殖能力,矿化诱导后茜素红染色观察矿化结节的形成情况,碱性磷酸酶(ALP)活性检测和实时定量聚合酶链反应(RT-PCR)检测成骨相关基因表达。结果 MTT检测结果显示,H组OD值较L组和C组低(P0.05);成骨诱导21 d后,H组矿化结节面积较L组和C组少(P0.05);成骨诱导期间,H组ALP活性较L组和C组明显偏低(P0.05);H组早期成骨基因Runt相关转录因子2、ALP和Ⅰ型胶原诱导前后相对倍增数较L组和C组低(P0.05)。结论高浓度葡萄糖能够抑制PDLC增殖能力和成骨分化能力。
[Abstract]:Objective to study the proliferation and osteogenic differentiation of periodontal ligament fibroblasts (PDLC) stimulated by high glucose concentration. PDLC was stimulated by glucose in 0 mg 路L ~ (-1) C group (n = 1 100 mg 路L ~ (-1)) L group (n = 4 500 mg 路L ~ (-1)). The proliferative ability of PDLC was detected by thiazolyl blue method, and the formation of mineralized nodules was observed by alizarin red staining after mineralization induction. Alkaline phosphatase (ALP) activity and real-time quantitative polymerase chain reaction (RT-PCR) were used to detect the expression of osteoblast-associated genes. The OD value of group H was lower than that of group L and group C (P 0.05). 21 days after osteogenesis induction, the area of mineralized nodules in group H was less than that in group L and group C (P 0.05). During osteogenic induction, the activity of ALP in group H was significantly lower than that in group L and group C (P 0.05). Group H had early osteogenic gene Runt related transcription factor 2. The relative doubling number of ALP and collagen I before and after induction was lower than that of group L and group C. ConclusionHigh concentration of glucose can inhibit the proliferation and osteogenic differentiation of PDLC.
【作者单位】：浙江大学医学院附属第二医院综合牙科;重庆医科大学附属口腔医院牙体牙髓病科重庆市口腔疾病与生物医学重点实验室;浙江大学医学院附属第二医院口腔矫形科;
【分类号】：R781.4
【正文快照】： 在牙周膜成纤维细胞(periodontal?ligament?cell,PDLC)中,包含着少量具有自我更新能力和多项分化潜能的间充质来源的牙周膜干细胞(pe-riodontal?ligament?stem?cell,PDLSC),PDLSC具有干细胞的特性,可形成骨样结构和成牙本质样结构,对牙周组织的缺损有明显的修复作用[1-2]。由

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