正畸牙移动过程中尼古丁摄入对牙周组织影响

发布时间：2018-02-07 13:25

本文关键词：尼古丁正畸学环氧化酶牙骨质组织构建组织工程尼古丁酒石酸溶液正畸牙移动免疫组织化学原位杂交环氧化酶mRNA 破牙骨质细胞牙根吸收牙周组织　出处：《中国组织工程研究》2015年46期 　论文类型：期刊论文

【摘要】：背景:吸烟是严重影响牙周组织和牙根健康的危险因素之一,烟草中的尼古丁会加速牙周病患者牙周组织的破坏。目的:分析正畸牙移动过程中不同剂量尼古丁作用下牙周组织内环氧化酶2及mR NA表达的变化规律。方法:将110只SD大鼠随机分为5组,空白对照组10只,生理盐水组和尼古丁0.5,0.75,1 mg/kg组各25只。除空白组外所有大鼠使用50 g力牵拉一侧上颌第1磨牙向近中移动。生理盐水组每日腹腔注射0.1 mL生理盐水;尼古丁组每日腹腔注射0.5,0.75,1 mg/kg尼古丁酒石酸溶液。各组大鼠分别在加力1,3,5,7,14 d时处死取上颌组织,苏木精-伊红染色观察牙周组织变化,免疫组织化学染色计数破牙骨质细胞阳性细胞,原位杂交染色法检测环氧化酶2在牙周组织中表达的平均吸光度值。结果与结论:尼古丁各剂量组在各加力时间点破牙骨质细胞数目均高于未注射尼古丁组,且环氧化酶2阳性细胞表达强度也均高于未注射尼古丁组。随尼古丁注射剂量增大,破牙骨质细胞数目也逐渐增加(P0.05),加力7 d时各组破牙骨质细胞数目均达到峰值;环氧化酶2阳性细胞表达强度也随尼古丁注射剂量增大而增强(P0.05),加力5 d时表达强度达到峰值。结果表明,在相同加力时间点,破牙骨质细胞数目随剂量增大而增多,且环氧化酶2阳性细胞表达强度也随剂量增加而增强。正畸牙移动过程中的尼古丁摄入会造成牙周组织的破环,且尼古丁摄入剂量的高低直接影响牙周组织的破坏程度。
[Abstract]:Background: smoking is one of the major risk factors for periodontal tissue and root health. Nicotine in tobacco can accelerate the destruction of periodontal tissue in patients with periodontal disease. Objective: to analyze the changes of cyclooxygenase-2 and mRNA expression in periodontal tissues under different doses of nicotine during orthodontic tooth movement. 110 SD rats were randomly divided into 5 groups. Ten rats in the blank control group, 25 rats in the saline group and 25 rats in the nicotine 0.5-0.75 mg/kg group. All the rats except the blank group were moved to the proximal part of the maxillary first molar with 50 g traction force. The saline group was intraperitoneally injected with 0.1 mL normal saline daily. The rats in the nicotine group were intraperitoneally injected with 0.5-0.75 mg/kg nicotine tartaric acid solution daily. The maxillary tissues were sacrificed at the time of 7d after treatment for 14 days, and the periodontal tissues were observed by hematoxylin and eosin staining, and the changes of the periodontal tissues were observed by hematoxylin and eosin staining. The positive cells of cementoclast cells were counted by immunohistochemical staining. The average absorbance of cyclooxygenase 2 expression in periodontal tissue was detected by in situ hybridization staining. Results and conclusion: the number of osteoclast cells in each dose of nicotine group was higher than that in the control group at different time points after administration of nicotine. The expression intensity of cyclooxygenase-2 positive cells was also higher than that of non-nicotine injection group. With the increase of nicotine injection dose, the number of osteoclast cells increased gradually, and the number of cementoclast cells reached the peak value at 7 days. The expression intensity of cyclooxygenase-2 positive cells also increased with the increase of nicotine injection dose, and reached a peak at 5 days after administration. The results showed that the number of cementoclast cells increased with the increase of dose at the same time point. Moreover, the expression of cyclooxygenase-2 positive cells also increased with the increase of dose. Nicotine intake during orthodontic tooth movement would cause the periodontal tissue to break, and the dose of nicotine intake directly affected the degree of periodontal tissue damage.
【作者单位】：新疆医科大学第一附属医院口腔正畸科;新疆医科大学口腔医学院口腔正畸教研室;新疆库尔勒市农二师医院综合门诊部口腔科;
【基金】：新疆医学动物模型研究实验室开放课题基金资助(XJDX1103-2012-04),课题名称:尼古丁对正畸力作用下大鼠牙周组织改建的影响~~
【分类号】：R783.5

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