两种矿化胶原的显微结构对成骨样细胞MG 63形貌的影响

发布时间：2018-02-10 11:54

本文关键词： 矿物质胶原组织支架细胞黏附骨再生　出处：《北京大学学报(医学版)》2014年01期 　论文类型：期刊论文

【摘要】：目的:研究两种矿化胶原的显微结构对人成骨样细胞MG 63的黏附力和形貌的影响。方法:采用传统矿化法和生物仿生矿化法分别制备纤维外矿化胶原(extrafibrillarly-mineralized collagen,EMC)与纤维内矿化胶原(intrafibrillarly-mineralized collagen,IMC)支架材料,人成骨样细胞MG 63作为实验用细胞,用扫描电子显微镜(scanning electron microscopy,SEM)检测两种支架材料的显微结构及细胞与材料间相互作用。采用激光共聚焦显微镜(laser scanning microscope,LSM)检测接种在材料上细胞的黏附力与形貌。结果:不同矿化方式影响胶原的显微结构。EMC组可见花样团簇状的晶体无规则沉积在纳米纤维束表面,而IMC组纤维束表面光滑,无晶体沉积。能谱分析结果显示其内部含有晶体,为含碳的钙缺乏型羟基磷灰石,这与天然骨矿化胶原成分类似。LSM结果显示,IMC比EMC支架材料更能促进MG 63细胞伸展。荧光定量分析结果表明MG 63细胞对IMC支架材料的黏附力(18.54±2.71)明显高于EMC支架材料(14.29±1.32)。SEM结果显示两种矿化胶原均具有良好的生物相容性,细胞在不同支架上呈现不同的形貌。结论:矿化胶原的显微结构影响细胞的形貌,IMC支架材料更能促进MG63细胞的黏附与伸展,这将有助于研制新的牙槽骨再生的生物仿生材料。
[Abstract]:Objective: to study the effect of microstructures of two kinds of mineralized collagen on adhesion and morphology of human osteoblast-like cell MG63. Methods: extracellular mineralized collagen (EMCMC) and fibers were prepared by traditional mineralization method and biomimetic mineralization method, respectively. Intrafibrillarly-collagenized IMCC-based scaffold material, Human osteoblast-like cell MG63 was used as experimental cell. Scanning electron microscopy (SEM) was used to detect the microstructure of the two scaffolds and the interaction between cells and materials. The adhesion and morphology of the cells inoculated on the materials were detected by laser confocal microscopy (LSM). The microstructures of collagen were affected by different mineralization modes. In EMC group, irregular crystals were deposited on the surface of nanofilament bundles. In IMC group, the surface of fiber bundle is smooth and there is no crystal deposition. The results of energy spectrum analysis show that the fiber bundle contains crystal, which is calcium deficient hydroxyapatite. The results of fluorescence quantitative analysis showed that the adhesion of MG63 cells to IMC scaffolds was significantly higher than that of EMC scaffolds, and the fluorescence quantitative analysis showed that the adhesion of MG63 cells to IMC scaffolds was significantly higher than that of EMC scaffolds. The results of SEM showed that the two mineralized collagen had good biocompatibility. Conclusion: the microstructure of mineralized collagen can affect the morphology of MG63 cells, which can promote the adhesion and extension of MG63 cells, which will be helpful for the development of new biomimetic materials for alveolar bone regeneration.
【作者单位】：北京大学口腔医学院·口腔医院正畸科;
【基金】：国家自然科学基金(81201198)资助~~
【分类号】：R783.1

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