线粒体在埃克替尼诱导涎腺腺样囊性癌细胞系ACC-M细胞凋亡中的作用

发布时间：2018-02-10 15:27

本文关键词： 埃克替尼涎腺腺样囊性癌细胞线粒体凋亡　出处：《安徽医科大学学报》2014年10期 　论文类型：期刊论文

【摘要】：目的利用涎腺腺样囊性癌细胞系ACC-M细胞为模型,研究不同浓度埃克替尼(icotinib)对涎腺腺样囊性癌细胞系ACC-M细胞凋亡的影响以及线粒体在icotinib诱导ACC-M细胞凋亡中的作用。方法 icotinib和P53抑制剂(PFT)分别处理ACC-M细胞,应用噻唑蓝比色法检测细胞活性,利用Western blot法检测P53和胞质内细胞色素C(Cyt C)蛋白的表达变化,用Caspase-3凋亡相关蛋白活力检测ACC-M细胞的凋亡情况。结果 icotinib可抑制ACC-M细胞的活性,增加胞质Cyt C蛋白的表达和P53在线粒体的转位并诱导ACC-M细胞凋亡。用PFT预处理后,icotinib诱导ACC-M细胞的凋亡明显下降,线粒体蛋白P53的表达降低,胞质内Cyt C表达减少,Caspase-3凋亡相关蛋白活力下降。结论 icotinib可能通过诱导P53转位到线粒体进而促进ACC-M细胞凋亡。
[Abstract]:Objective to establish a model of salivary adenoid cystic carcinoma cell line ACC-M. To study the effects of different concentrations of ectinimide on apoptosis of ACC-M cells and the role of mitochondria in icotinib induced apoptosis of ACC-M cells. Methods ACC-M cells were treated with icotinib and p53 inhibitor PFTs, respectively. The activity of ACC-M cells was detected by thiazolyl blue colorimetry, the expression of p53 and cytochrome C _ (Cyt C) protein was detected by Western blot assay, and the apoptosis of ACC-M cells was detected by Caspase-3 apoptosis-related protein activity. Results icotinib could inhibit the activity of ACC-M cells. The expression of cytoplasmic Cyt C protein and p53 translocation in mitochondria were increased and apoptosis of ACC-M cells was induced. After pretreatment with PFT, the apoptosis of ACC-M cells was significantly decreased, and the expression of mitochondrial protein p53 was decreased. The expression of Cyt C in cytoplasm decreased the activity of apoptosis-related protein of Caspase-3. Conclusion icotinib may promote apoptosis of ACC-M cells by inducing p53 translocation to mitochondria.
【作者单位】：新乡医学院第一附属医院口腔外科;新乡医学院人体解剖学教研室;新乡医学院第三附属医院消化内科;郑州大学口腔医学院口腔病理教研室;
【基金】：河南省教育厅科学技术研究重点项目(编号:14B320018)
【分类号】：R739.86

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