低温等离子体活性水对致病菌的杀灭作用研究

发布时间：2018-02-12 01:58

本文关键词： 低温等离子体低温等离子体活性水致病菌生物膜活性氧　出处：《兰州大学》2014年硕士论文　论文类型：学位论文

【摘要】：低温等离子体因其可以有效的杀灭存在于空气中以及液体中的致病菌,而逐渐被众多学者所认可。低温等离子体活性水作为低温等离子体一种新的杀菌形式出现,其杀菌作用以及杀菌机理还处于初步阶段,这需要我们进一步去研究发现。目的：通过低温等离子体活性水处理金黄色葡萄球菌、大肠杆菌、变形链球菌、白色念珠菌及变形链球菌生物膜,探讨低温等离子体活性水的杀菌效应以及杀菌机理。方法：通过直流大气压Ar/O2(2%)低温等离子体处理20mL蒸馏水20min,来制备低温等离子体活性水。制备完成的低温等离子体活性水即刻处理金黄色葡萄球菌,大肠杆菌,变形链球菌,白色念珠菌和变形链球菌生物膜。菌落形成单位数和荧光染色评价低温等离子体活性水的杀菌效果。用扫描电子显微镜观察变形链球菌游离菌和变形链球菌生物膜的表面形态结构。共聚焦激光扫描显微镜用来显示低温等离子体活性水对变形链球菌生物膜的杀菌效果。过氧化氢检测试剂盒和发射光谱仪检测低温等离子体活性水中的活性物质,并分析讨论低温等离子体活性水的杀菌机理。结果：菌落计数法、荧光染色法的检测结果：低温等离子体活性水在20min内可以有效的杀灭金黄色葡萄球菌、大肠杆菌和变形链球菌,对白色念珠菌及变形链球菌生物膜的杀灭作用甚微；扫描电子显微镜显示我们成功建立了变形链球菌生物膜模型,并通过共聚焦激光扫描显微镜观测到生物膜的三维立体结构；在波长为777.2nm处可以清楚地观察到原子氧的存在,并在低温等离子体活性水中检测到浓度为3.0mg/L的过氧化氢,而这些活性物质可能在PAW杀菌过程中起着不同的作用. 结论：低温等离子体活性水可以有效的杀灭细菌(金黄色葡萄球菌、大肠杆菌和变形链球菌),但是对真菌以及变形链球菌生物膜的杀菌作用较弱。
[Abstract]:Low temperature plasma has been recognized by many scholars because it can effectively kill pathogenic bacteria in air and liquid. Low temperature plasma active water as a new form of sterilization of low temperature plasma appears. Its bactericidal action and mechanism are still in the preliminary stage, which needs to be further studied and discovered. Objective: to study the bactericidal effect and bactericidal mechanism of low temperature plasma active water in treating the biofilms of Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Candida albicans and Streptococcus mutans. Methods: 20 mL distilled water was treated with low temperature plasma at DC atmospheric pressure for 20 min to prepare low temperature plasma active water. The finished low temperature plasma active water was immediately treated with Staphylococcus aureus, Escherichia coli, Streptococcus mutans. Candida albicans and Streptococcus mutans biofilms. Colony formation units and fluorescent staining to evaluate the bactericidal efficacy of low temperature plasma active water. Observation of streptococcus mutans free bacteria and Streptococcus mutans by scanning electron microscopy. Surface morphology of the film. Confocal laser scanning microscopy is used to show the bactericidal effect of low temperature plasma active water on Streptococcus mutans biofilms. Hydrogen peroxide detection kit and emission spectrometer for low temperature plasma detection. Active substances in active water, The bactericidal mechanism of low temperature plasma active water was analyzed and discussed. Results: the results of colony counting and fluorescent staining showed that low temperature plasma active water could effectively kill Staphylococcus aureus, Escherichia coli and Streptococcus mutans within 20 minutes. The biofilm of Candida albicans and Streptococcus mutans had little effect on killing, scanning electron microscope showed that we had successfully established the biofilm model of Streptococcus mutans, and the three-dimensional structure of biofilm was observed by confocal laser scanning microscope. The existence of atomic oxygen can be clearly observed at 777.2 nm and the concentration of hydrogen peroxide is 3.0 mg / L in low temperature plasma active water. These active substances may play different roles in the sterilization of PAW. Conclusion: low temperature plasma active water can effectively kill bacteria (Staphylococcus aureus, Escherichia coli and Streptococcus mutans), but the bactericidal effect on fungi and Streptococcus mutans biofilms is weak.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R780.2

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