血清IgG抗体含量与口腔厌氧菌致牙髓感染的关系

发布时间：2018-02-12 05:54

本文关键词： IgG抗体 ELISA方法牙髓感染口腔厌氧菌　出处：《中华医院感染学杂志》2015年16期 　论文类型：期刊论文

【摘要】：目的利用抗原与抗体特异反应的特点,通过检测血清抗体IgG水平分析口腔厌氧菌与牙髓感染的关系,以降低感染率。方法将2013年1月-2014年1月患有不同牙髓疾病的患者分为B、C、D组,每组各10例,另选10名健康受试者为A组;应用ELISA方法测定健康受试组与感染组患者的血清中10株国际口腔厌氧标准菌株的IgG抗体含量,特异性分析牙髓感染与口腔厌氧菌的关系,数据采用SPSS 17.0软件进行统计分析。结果健康受试组的中间普氏菌血清抗体平均值显著低于B、C、D组(P0.05);且各组OD值与A组健康受试者相比2.1,全部为阳性;健康受试组的牙龈卟啉单胞菌血清抗体平均值与牙髓感染组、牙髓-牙周联合病症组患者差异有统计学意义(P0.05),但与牙周疾病组患者相比较,差异无统计学意义;各组厌氧菌血清抗体OD值之间差异均无统计学意义。结论通过ELISA方法分析与牙髓感染相关的厌氧菌,此种方法无需微生物培养,且其检出率及精确度远高于传统的微生物培养法,可作为临床上检测牙髓感染致病菌的常规方法。
[Abstract]:Objective to analyze the relationship between oral anaerobes and dental pulp infection by detecting the serum antibody IgG level based on the specific reaction of antigen and antibody. Methods the patients with different dental pulp diseases from January 2013 to January 2014 were divided into two groups: group B (n = 10) and group A (n = 10). ELISA method was used to determine the IgG antibody content of 10 international oral anaerobic standard strains in the serum of healthy subjects and infected patients, and the relationship between dental pulp infection and oral anaerobes was analyzed. The data were statistically analyzed by SPSS 17.0 software. Results the average value of serum antibodies against PPD in the healthy subjects was significantly lower than that in the control group (P 0.05), and the OD value in each group was 2.1 compared with the healthy subjects in group A, all of which were positive. There was a significant difference in serum antibody between healthy subjects and dental pulp infection group and pulp-periodontal disease group (P 0.05), but there was no significant difference compared with periodontal disease group. There was no significant difference between the OD values of serum antibodies in each group. Conclusion the ELISA method is used to analyze the anaerobic bacteria associated with dental pulp infection, and no microbial culture is required for this method. Its detection rate and accuracy are much higher than the traditional microbial culture method, which can be used as a routine method to detect the pathogenic bacteria of dental pulp infection.
【作者单位】：天津市口腔医院牙体牙髓一科;
【基金】：天津市科技局研究基金资助项目(1203684)
【分类号】：R780.2

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