不同咀嚼负荷对幼兔髁突软骨内印度豪猪蛋白-人甲状旁腺激素相关蛋白通路表达的影响

发布时间：2018-02-23 20:35

本文关键词： 髁突软骨咀嚼力印度豪猪蛋白甲状旁腺激素相关蛋白　出处：《华西口腔医学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的分析不同咀嚼负荷作用下,幼兔髁突软骨内印度豪猪蛋白(Ihh)-人甲状旁腺激素相关蛋白(PThrP)通路表达的差异性,探讨咀嚼应力负荷对髁突软骨Ihh-PThrP信号通路的影响。方法选取10d龄幼兔48只,随机分为硬食组和软食组,分别喂以同种颗粒状(硬食)和粉状(软食),于饲养的第2、4、6、8周处死。采用苏木精-伊红(HE)染色、免疫组织化学、免疫印迹、实时定量荧光聚合酶链反应检测Ihh和PThrPmRNA和蛋白的动态变化。结果HE染色显示硬食组髁突软骨厚度高于软食组的厚度;第2、4、6、8周,Ihh蛋白、PThrP蛋白和mRNA表达量在两组中呈递减趋势;软食组中Ihh和PThrP蛋白以及mRNA表达量显著低于硬食组。结论较低的咀嚼负荷会造成髁突软骨生长因子Ihh和PThrP分泌减少,Ihh-PThrP通路表达迟缓,软骨发育受阻碍;适当的咀嚼负荷对髁突的正常发育有至关重要的作用。
[Abstract]:Objective to analyze the different chewing load, India hedgehog rabbit mandibular condylar cartilage (Ihh) - human parathyroid hormone related protein (PThrP) expression of different pathways, chewing the effect of stress load on the condylar cartilage of Ihh-PThrP signaling pathway. Methods 10d instar larvae of 48 rabbits were randomly divided into hard food group and diet group were fed with the same granular (hard food) and powder (ruanshi), in the 2,4,6,8 weeks. Raised by hematoxylin eosin (HE) staining, immunohistochemistry, Western blot and real-time quantitative polymerase chain reaction to detect Ihh and PThrPmRNA protein and HE staining. Results show the hard diet group condylar cartilage thickness is higher than the thickness of the soft group; the 2,4,6,8 week, Ihh protein content in two groups decreased the expression of PThrP and mRNA; Ihh and PThrP in soft diet group and mRNA protein expression was significantly lower than the hard diet group. Conclusion low The masticatory load will decrease the secretion of growth factor Ihh and PThrP in condylar cartilage, slow down the expression of Ihh-PThrP pathway, and block the development of cartilage. Proper masticatory load plays a crucial role in the normal development of condyle.

【作者单位】：浙江中医药大学口腔医学院;
【基金】：国家自然科学基金(81200802) 浙江省自然科学基金(Y2111273)~~
【分类号】：R78

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