牙周治疗前后龈沟液中Ⅰ型胶原羧基端肽及钙卫蛋白的研究

发布时间：2018-02-23 22:27

本文关键词： 龈沟液 Ⅰ型胶原羧基端肽钙卫蛋白 ELISA　出处：《山东大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的： I型胶原羧基端肽(pyridinoline crossed-linked carboxyterminal telopeptide of type I collagen,ICTP)是骨吸收的特异性标志物，研究表明龈沟液中ICTP的含量可反映牙周炎患者牙槽骨的破坏程度。钙卫蛋白(calprotectin,S100A8/A9complex)被认为是炎症性疾病的标志,其在龈沟液中的高表达与牙周炎的炎症程度密切相关。本研究的目的是通过检测龈沟液中这两种成分的含量在牙周基础治疗前后的变化探讨它们作为牙周炎诊断和疗效评价的生化指标的可行性。方法：选取14例慢性牙周炎患者作为实验组,8例健康人作为对照组，，每人选择2颗磨牙的单个邻面位点为研究对象。采用吸潮纸尖收集治疗前、基础治疗1周后同一位点及健康牙的龈沟液,称取龈沟液的重量,记录牙龈指数、牙周袋深度、附着丧失水平,采用ELISA法检测龈沟液中ICTP和S100A8/A9的浓度,分别对三组结果及临床指标进行统计学分析。结果： 1.牙周炎组治疗前龈沟液中ICTP、 S100A8/A9浓度分别大于对照组浓度,差别具有统计学意义(P0.01)。 2.治疗前龈沟液中ICTP、 S100A8/A9浓度与牙龈指数、牙周袋深度、附着丧失均呈正相关关系(P0.05)。 3.经基础治疗1周后,龈沟液中ICTP、 S100A8/A9浓度较治疗前明显降低,但仍高于对照组,差别具有统计学意义(P0.01)。 4.牙周炎组治疗后牙龈指数、牙周袋深度、附着丧失均小于治疗前,差别具有统计学意义(P0.01)。 5.治疗前、后龈沟液中ICTP与S100A8/A9的浓度均呈线性正相关关系(P0.01)。结论：慢性牙周炎患者龈沟液中ICTP S100A8/A9浓度明显高于健康人,且与临床指标呈正相关,经过牙周基础治疗后二者浓度明显下降,治疗前后ICTP与S100A8/A9的浓度均呈明显正相关。该结果表明龈沟液中ICTP. S100A8/A9的含量可作为牙周炎诊断的辅助指标,并能有效评价基础治疗的疗效。
[Abstract]:Objective:. Type I collagen carboxyl terminal peptide pyridinoline crossed-linked carboxyterminal telopeptide of type I collagenesis (ICTP) is a specific marker of bone resorption. Studies have shown that the content of ICTP in gingival crevicular fluid can reflect the degree of alveolar bone destruction in patients with periodontitis. Calprotectinine S100A8 / A9 complex is considered a marker of inflammatory disease. Its high expression in gingival crevicular fluid was closely related to the degree of periodontitis. The purpose of this study was to investigate the changes of these two components in gingival crevicular fluid before and after periodontal basic treatment. And the feasibility of biochemical indicators for efficacy evaluation. Methods:. Fourteen patients with chronic periodontitis were selected as the control group (n = 8). The single adjacent surface of 2 molars was selected as the control group. Gingival crevicular fluid (GCF) of the same site and healthy teeth was measured after 1 week of basic treatment. The gingival index, the depth of periodontal bag and the level of attachment loss were recorded. The concentrations of ICTP and S100A8 / A9 in gingival crevicular fluid were measured by ELISA method. The results and clinical indexes of the three groups were analyzed statistically. Results:. 1. The concentrations of ICTP, S100A8 / A9 in gingival crevicular fluid before treatment in periodontitis group were higher than those in control group (P 0.01). 2. The concentrations of ICTP, S100A8 / A9 in gingival crevicular fluid were positively correlated with gingival index, periodontal pocket depth and attachment loss before treatment. 3. After one week of basic treatment, the concentrations of ICTPand S100A8 / A9 in gingival crevicular fluid were significantly lower than those before treatment, but still higher than those in the control group (P 0.01). 4. The gingival index, the depth of periodontal bag and the loss of attachment after treatment in periodontitis group were lower than those before treatment, and the difference was statistically significant (P 0.01). 5. Before treatment, the concentration of ICTP and S100A8 / A9 in gingival crevicular fluid was positively correlated with that of S100A8 / A9. Conclusion:. The concentration of ICTP S100A8 / A9 in gingival crevicular fluid in patients with chronic periodontitis was significantly higher than that in healthy subjects, and was positively correlated with clinical indexes. ICTP was positively correlated with the concentration of S100A8 / A9 before and after treatment. The results showed that the contents of ICTP. S100A8 / A9 in gingival crevicular fluid could be used as an auxiliary index for the diagnosis of periodontitis and the curative effect of basic treatment could be evaluated effectively.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.4

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