不同浓度雌激素对人源性牙周膜干细胞成骨分化能力的影响

发布时间：2018-02-24 18:01

本文关键词： 牙周膜干细胞成骨分化雌激素　出处：《浙江中西医结合杂志》2013年12期 　论文类型：期刊论文

【摘要】：目的:对比研究不同浓度的雌激素对人源性牙周膜干细胞成骨分化的影响。方法:采用DMEM完全培养基将人牙周膜干细胞制成单细胞悬液,分别加入浓度为0、10×10-10、10×10-9、10×10-8、10×10-7、10×10-6、10×10-5mol/L的雌激素,以未加入雌激素的成骨诱导液组(普通DMEM培养基中加入10mMβ-甘油磷酸钠、50μM维生素C及10nM地塞米松)作为阴性对照。培养后实时定量PCR方法分别检测成骨早中晚期关键指标Runx2、ALP和OCN mRNA表达。结果:浓度为10×10-10、10×10-9、10×10-8、10×10-7、10×10-6、10×10-5mol/L的雌激素对人源性的牙周膜干细胞培养均无毒性;不同浓度组间差异无统计学意义(P0.05);在成骨诱导7、14、21天时,Runx2、ALP及OCN mRNA表达在10×10-7组最高(P0.05～0.01)。在不同浓度雌激素组中成骨相关基因的表达均高于阴性对照组(P0.05)。结论:当雌激素浓度10×10-7mol/L时,雌激素能够以剂量依赖的方式促进人牙周膜干细胞成骨分化过程中成骨相关基因的表达。当雌激素浓度10×10-7mol/L时,雌激素浓度的增加并未促进成骨相关基因的表达。
[Abstract]:Objective: to compare the effects of different concentrations of estrogen on the osteogenic differentiation of human periodontal ligament stem cells. Methods: human periodontal ligament stem cells were prepared into single cell suspension using DMEM complete medium, and 10 脳 10 ~ (-9) 10 脳 10 ~ (-9) 10 脳 10 ~ (-8) 10 脳 10 ~ (-7) C ~ 10 脳 10 ~ (-6) 10 脳 10 ~ (-5) mol 路L ~ (-1) estrogen. The osteogenic induction solution without estrogen (10 mm 尾 -glycerophosphate 50 渭 M vitamin C and 10 nm dexamethasone) was used as negative control in normal DMEM medium. Real time quantitative PCR was used to detect Guan in early, middle and late stage of osteogenesis. Results: 10 脳 10 -10 ~ (-9) 10 脳 10 ~ (-9) 10 脳 10 ~ (-8) 10 脳 10 ~ (-8) 10 脳 10 ~ (-7) 10 脳 10 ~ (-6) 10 脳 10 ~ (-5) mol / L estrogen was not toxic to human periodontal ligament stem cell culture. There was no significant difference between different concentration groups (P 0.05), but the expression of Runx2ALP and OCN mRNA in 10 脳 10 ~ (-7) group was the highest at 21 days after osteogenesis induction. The expression of osteoblast-related genes in different concentration estrogen group was higher than that in negative control group (P 0.05). Conclusion: when estrogen concentration is 10 脳 10 ~ (-7) mol / L, the expression of osteoblast-related gene in 10 脳 10 ~ (-7) mol / L group is higher than that in negative control group. Estrogen could promote the expression of osteoblast-associated genes during osteogenic differentiation of human periodontal ligament stem cells in a dose-dependent manner, but the increase of estrogen concentration did not promote the expression of osteoblast-associated genes when the concentration of estrogen was 10 脳 10 ~ (-7) mol / L.
【作者单位】：杭州市红十字会医院口腔科;
【分类号】：R780.2

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