紫杉醇类联合卡铂经不同途径治疗兔VX-2舌癌的实验研究

发布时间：2018-02-28 13:38

  本文关键词： 紫杉醇 紫杉醇脂质体 兔VX-2舌癌 动脉化疗 静脉化疗　出处：《遵义医学院》2014年硕士论文　论文类型：学位论文

【摘要】：目的：采用紫杉醇类联合卡铂经不同途径灌注治疗兔VX-2舌癌，比较疗效的差异。 方法：建立兔VX-2舌癌动物模型，随机分为6组，每组4只，分别为：A1（紫杉醇脂质体联合卡铂舌动脉灌注给药组），A2（紫杉醇联合卡铂舌动脉灌注给药组），A（葡萄糖舌动脉灌注给药组），V1（紫杉醇脂质体联合卡铂全身静脉给药组），，V2（紫杉醇联合卡铂全身静脉给药组），V（葡萄糖全身静脉给药组）。化疗给药时间1小时。化疗1周后通过测量舌癌原发灶体积、流式细胞术、免疫组化的方法评价肿瘤抑瘤率、细胞凋亡情况、p53蛋白表达情况，各组数据用SPSS20.0统计软件进行分析，比较紫杉醇类联合卡铂经不同给药途径治疗兔VX-2舌癌的疗效。 结果：①化疗后各组有效率分别为：A1组4/4、A2组1/4，V1、V2、A、V组0/4。A1组与V1组、V2组、A组、V组组间比较具有显著性差异（P0.05）；余组间比较无显著性差异（P0.05）。②经FCM检测肿瘤细胞凋亡率（%）：A1组：84.80±3.35、A2组：62.70±7.92、A组：8.63±0.38、V1组：52.05±3.96、V2组：29.45±0.15、V组：8.61±0.28，A1组明显高于其它各组，各组间比较具有显著性差异（P0.05）。③免疫组化测得P53蛋白在肿瘤组织的表达：在A1、A2、V1、V2、A、V的阳性表达率分别为8.33%、33.33%、58.33%、66.67%、83.33%、83.33%。A1组分别与V1组、V2组、A组、V组组间比较具有显著性差异（P0.05）；A2组分别与A组、V组组间比较具有显著性差异（P0.05）；余各组间比较无显著性差异（P0.05）。 结论：①紫杉醇脂质体联合卡铂化疗较紫杉醇联合卡铂治疗兔VX-2舌癌近期疗效显著；②经舌动脉灌注化疗较全身静脉化疗治疗兔VX-2舌癌近期疗效显著。
[Abstract]:Objective: To compare the therapeutic effect of paclitaxel and carboplatin through different ways in the treatment of VX-2 tongue cancer in rabbits.
Methods: rabbit tongue VX-2 animal model, randomly divided into 6 groups, 4 rats in each group, respectively: A1 (lingual arterial infusion of paclitaxel liposome combined with Carboplatin Injection group (A2), lingual artery perfusion with paclitaxel and carboplatin administration group (A), glucose tongue arterial infusion group), V1 (paclitaxel liposome combined with carboplatin in the intravenous group), V2 (paclitaxel combined with carboplatin in the intravenous group), V (glucose intravenous group). Chemotherapy was administered for 1 hours. After 1 weeks of chemotherapy by measuring the volume of primary squamous cell carcinoma of the tongue, flow cytometry, method of immunohistochemical evaluation of tumor inhibition rate, cell apoptosis, the expression of p53 protein were analyzed by SPSS20.0 statistical software, all the data were compared with paclitaxel and carboplatin in the curative effect of different routes of Administration for the treatment of rabbit VX-2 carcinoma of tongue.
缁撴灉锛氣憼鍖栫枟鍚庡悇缁勬湁鏁堢巼鍒嗗埆涓猴細A1缁

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