女性正畸患者月经周期中最佳加力时机的初步研究

发布时间：2018-03-01 09:28

本文关键词： 龈沟液雌激素正畸力骨保护素　出处：《华西口腔医学杂志》2014年03期 　论文类型：期刊论文

【摘要】：目的通过在女性月经周期中的不同时期对正畸牙加力,研究龈沟液内雌激素(E2)、骨钙素(OCN)、核因子κB受体活化因子配体(RANKL)、骨保护素(OPG)的时效性表达,从而为临床选择最佳加力时机提供理论依据。方法选取18~28周岁双侧对称拔除第一恒前磨牙青年女性恒牙患者12人。随机选取其中6人为月经期加力组,6人为排卵期加力组,于右侧尖牙单侧使用镍钛拉簧施加1.5 N正畸力使之远中移动,对侧尖牙不做处理。使用牙周滤纸分别于加力前,加力后15、30、45 d取右侧尖牙远中(压力侧)龈沟液样本。使用化学发光法测定龈沟液中E2、OCN的活性水平。使用酶联免疫法检测龈沟液中RANKL及OPG的活性水平。结果排卵期加力组龈沟液E2和OCN水平高于月经期加力组(P0.05)。月经期加力组与排卵期加力组中RANKL水平与OPG水平及RANKL/OPG比值均未见明显差异(P0.05)。结论女性正畸拔牙患者于雌激素水平较高的排卵期施加正畸力,骨保护因子E2、OCN表达增强,不利于牙槽骨改建。而于月经期加力,上述因子表达减弱,可能有助于牙齿快速移动。
[Abstract]:Objective to study the time-dependent expression of estrogen in gingival crevicular fluid (E2N), osteocalcin (OCNN), nuclear factor- 魏 B receptor activating factor ligand (RANKL) and osteoprotegerin (OPG) in female patients with orthodontic teeth at different periods of menstrual cycle. Methods 12 young female permanent teeth with first permanent premolars were selected for bilateral symmetrical extraction of first permanent premolars at the age of 18 ~ 28. Six of them were randomly selected as menstruating plus force group and 6 ovulatory postnatal patients. Apply 1.5 N orthodontic force on one side of right canine teeth to make them move far away, while contralateral canine teeth are not treated. Use periodontal filter paper before adding force, Gingival crevicular fluid (GCF) samples were collected from right canine distal (pressure side) on day 15, 30 and 45. The activity of E2OCN in gingival crevicular fluid was determined by chemiluminescence method. The activity of RANKL and OPG in gingival crevicular fluid was detected by enzyme linked immunosorbent assay (Elisa). The levels of E _ 2 and OCN in gingival crevicular fluid in the treatment group were higher than those in the postmenopausal group (P 0.05). There was no significant difference in the levels of RANKL and OPG and RANKL/OPG ratio between the two groups. Conclusion the estrogen level of female patients with orthodontic tooth extraction is higher than that of female patients with orthodontic tooth extraction. High ovulatory exertion of orthodontic force, The increased expression of osteoprotection factor E2OCN is not conducive to alveolar bone remodeling. However, during the menstrual period, the expression of these factors decreases, which may contribute to the rapid movement of teeth.
【作者单位】：重庆医科大学附属口腔医院正畸科;口腔疾病与生物医学重庆市重点实验室;
【基金】：重庆市自然科学基金资助项目(cstc2011jjA0046)
【分类号】：R783.5

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