明胶海绵复合生长因子加速颌骨骨折愈合的实验研究

发布时间：2018-03-03 20:32

本文选题：颌骨　切入点：骨折愈合　出处：《华西口腔医学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探索明胶海绵复合生长因子缓释系统加速颌骨骨折愈合的作用和机制,为临床加速颌骨骨折愈合提供新的方法。方法按每100μg基因重组人骨形态发生蛋白(BMP)-2用1 m L重组牛碱性成纤维细胞生长因子(b FGF)液完全溶解后,取40μL滴加到明胶海绵(0.5 cm×0.5 cm×1.0 cm)组织块中,冻干后制成b FGF/BMP/明胶海绵缓释系统。在12只新西兰大白兔两侧下颌制造线样骨折,左侧为对照组,只用钛板固定;右侧为实验组,钛板下放置b FGF/BMP/明胶海绵。术后2、4、12周行大体观察、X线检查、组织学检查。结果术后2周,实验组较对照组在骨折断端处可见更多纤维组织长入;术后4周,实验组骨折间隙可见到纤维性骨痂,对照组可见纤维组织和血管长入;12周后实验组和对照组骨折均已完全愈合。结论 b FGF/BMP/明胶海绵能加速骨折愈合,提高骨折愈合效果。
[Abstract]:Objective to explore the effect and mechanism of gelatin sponge combined with growth factor sustained release system in accelerating the healing of jaw fracture. Methods BMP-2 recombinant human bone morphogenetic protein per 100 渭 g gene was completely dissolved in 1 mL recombinant bovine basic fibroblast growth factor (BGF) solution. 40 渭 L drops were added to the tissue mass of gelfoam (0.5 cm 脳 0.5 cm 脳 1.0 cm), and the b FGF/BMP/ gelatin sponge sustained release system was prepared after lyophilization. Linear fractures were made on both sides of the mandible of 12 New Zealand white rabbits. The left side was the control group, only titanium plate was used, and the right side was the experimental group, and the right side was the experimental group. B FGF/BMP/ gelatin sponge was placed under titanium plate. Gross observation X-ray and histological examination were performed at 12 weeks after operation. Results at 2 weeks after operation, more fibrous tissue was observed in the experimental group than in the control group, 4 weeks after the operation, 4 weeks after the operation, more fibrous tissue was observed at the end of the fracture in the experimental group than in the control group. The fibrous callus could be seen in the fracture space of the experimental group, and the fibrous tissue and blood vessel in the control group were all healed completely after 12 weeks. Conclusion the b#en0# gelatin sponge can accelerate the fracture healing and improve the fracture healing effect.
【作者单位】：包头市中心医院口腔颌面外科;灵武市人民医院口腔科;
【基金】：宁夏银川市科技攻关课题(2008061)~~
【分类号】：R782.4

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