建立大鼠双膦酸盐相关颌骨骨坏死模型并初步分析其发病原因

发布时间：2018-03-05 08:00

本文选题：双膦酸盐相关颌骨骨坏死　切入点：模型　出处：《北京大学学报(医学版)》2014年06期 　论文类型：期刊论文

【摘要】：目的:建立大鼠双膦酸盐相关颌骨骨坏死(bisphosphonate-related osteonecrosis of the jaw,BRONJ)模型并初步分析其发病原因。方法:用帕米磷酸二钠、地塞米松加牙槽骨创伤(拔牙)的方法在大鼠体内建立了双膦酸盐相关颌骨骨坏死模型,并分析了CD4+和CD90+T淋巴细胞在双膦酸盐相关颌骨骨坏死发病过程中的变化。体外实验利用CCK-8法研究帕米磷酸二钠和地塞米松对人成骨肉瘤细胞MG-63和破骨前体细胞Raw 264.7的作用,初步分析双膦酸盐相关颌骨骨坏死的发病机制。结果:在牙槽骨创伤的前提下,成功地在帕米磷酸二钠和地塞米松联合用药组建立了大鼠BRONJ模型,流式细胞仪分析证实联合使用帕米磷酸二钠和地塞米松比单独应用这两种药物更能降低CD4+和CD90+T淋巴细胞在脾细胞中的比例。CCK-8实验证实帕米磷酸二钠能抑制MG-63及Raw264.7细胞的增殖,地塞米松能增强帕米磷酸二钠对抑制Raw 264.7细胞增殖的作用。结论:成功地建立了大鼠BRONJ模型,并证实了地塞米松可以增强双膦酸盐对破骨前体细胞抑制增殖作用;至少创伤、感染及地塞米松导致的免疫学反应等因素参与了BRONJ的发生和发展。
[Abstract]:Objective: to establish a bisphosphonate-related osteonecrosis of the jawbone Bronj model and analyze the pathogenesis of bisphosphonate-related osteonecrosis of the jawbone in rats. Dexamethasone plus alveolar bone trauma (extraction) method was used to establish a diphosphonate-associated osteonecrosis model in rats. The changes of CD4 and CD90 T lymphocytes in the pathogenesis of bisphosphonate-associated osteonecrosis of jaws were analyzed. The effects of disodium pamiphosphate and dexamethasone on human osteosarcoma cell line MG-63 and osteoclast Raw 264.7 were studied by CCK-8 method in vitro. Results: under the premise of alveolar bone trauma, the BRONJ model of rats was successfully established in the combination group of disodium Pamidophosphate and dexamethasone. Flow cytometry analysis showed that combined use of disodium Pamidophosphate and dexamethasone could lower the ratio of CD4 and CD90 T lymphocytes in splenocytes. CCK-8 assay showed that sodium Pamidophosphate could inhibit the proliferation of MG-63 and Raw264.7 cells. Dexamethasone can enhance the proliferation of Raw 264.7 cell line induced by disodium pamiphosphate. Conclusion: the rat BRONJ model was successfully established, and it was proved that dexamethasone could enhance the proliferation of osteoclastic precursor cells induced by bisphosphonates, at least in trauma. Infection and dexamethasone-induced immunological response are involved in the occurrence and development of BRONJ.
【作者单位】：北京大学口腔医学院·口腔医院特诊科;北京大学口腔医学院·口腔医院中心实验室;
【基金】：首都卫生发展科研专项基金(2011-4025-02)资助~~
【分类号】：R782.2

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