雷帕霉素通过抑制TLR4、IL-6、PGE2表达促进口腔鳞状细胞癌细胞凋亡研究

发布时间：2018-03-07 10:13

本文选题：口腔鳞状细胞　切入点：雷帕霉素　出处：《重庆医学》2017年28期 　论文类型：期刊论文

【摘要】：目的探讨雷帕霉素促进人口腔鳞状细胞癌SCC-15细胞凋亡是否与抑制TOLL样受体-4(TLR4)、白细胞介素(IL)-6、前列腺素E2(PGE2)表达有关。方法培养人口腔鳞状细胞癌SCC-15细胞株,雷帕霉素干预后,CCK-8法检测SCC-15细胞活性,Hochest33342/PI双染检测SCC-15细胞凋亡,Transwell法检测细胞侵袭能力,蛋白质印迹法(Western blot)检测TLR4蛋白表达水平,ELISA法检测IL-6、PGE2表达。结果雷帕霉素促进细胞凋亡,SCC-15细胞侵袭能力明显降低。雷帕霉素干预后,IL-6、PGE2水平降低,TLR4蛋白表达减少,与阴性组比较差异有统计学意义(P0.05)。结论雷帕霉素促进人口腔鳞状细胞癌细胞凋亡可能与抑制TLR4、IL-6、PGE2表达有关。
[Abstract]:Objective to investigate whether rapamycin can promote the apoptosis of human oral squamous cell carcinoma (SCC-15) cells by inhibiting the expression of TOLL like receptor -4TLR4, interleukin-6, prostaglandin E _ 2 (PGE2). Methods Human oral squamous cell carcinoma SCC-15 cell line was cultured. After the intervention of rapamycin, the activity of SCC-15 cells was detected by CCK-8 method. The apoptosis of SCC-15 cells was detected by double staining of Hochest33342% Pi and the ability of cell invasion was detected by Transwell method. Western blotting was used to detect the expression level of TLR4 protein and Elisa was used to detect the expression of IL-6pGE2.Results Rapamycin promoted the apoptosis of SCC-15 cells and the invasive ability of SCC-15 cells was significantly decreased, and the level of IL-6 / PGE2 decreased after rapamycin treatment, and the expression of TLR4 protein decreased after treatment with rapamycin. Conclusion the inhibitory effect of rapamycin on the apoptosis of human oral squamous cell carcinoma cells may be related to the inhibition of the expression of TLR4, IL-6 and PGE2.
【作者单位】：西南医科大学附属口腔医院牙周黏膜科;西南医科大学附属口腔医院检验科;
【基金】：四川省应用基础研究计划基金资助项目(2013JY0126) 四川省科技厅-泸州市科技局-西南医科大学联合科研基金资助项目(LY-62)
【分类号】：R739.8

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