骨保护素-聚乳酸羟基乙酸缓释微球的制备及体外释药性能研究

发布时间：2018-03-11 07:46

本文选题：骨保护素　切入点：聚乳酸羟基乙酸　出处：《安徽医科大学学报》2017年11期 　论文类型：期刊论文

【摘要】：目的以聚乳酸羟基乙酸(PLGA)为载体构建载有骨保护素(OPG)的微球,筛选出缓释效果最佳的制备条件,并研究载药微球的体外释放特性。方法采用复乳溶剂挥发法,以不同的搅拌速度、聚乙烯醇(PVA)浓度、PLGA浓度制备OPG-PLGA微球并测定其载药量和包封率,通过正交试验优化制备条件;以磷酸盐缓冲液作为释放介质考察载药微球的体外释放特性。结果以PLGA聚合物浓度400 mg/ml、搅拌速度400 r/min、PVA浓度2%为条件制备的载药微球具有最优的载药量和包封率,分别为6.21×10-7和75.10%,体外释药试验显示微球持续释放时间达到30 d,具有良好缓释效果。结论采用优化条件制备的OPG-PLGA微球具有较高的包封率和载药量,同时具有良好的缓释效果,为用于拔牙位点保存术的缓释药物研究提供了基础。
[Abstract]:Objective to construct the microspheres containing osteoprotegerin (OPG) with polylactic acid glycolate (PLGA) as the carrier, to select the best preparation conditions for the sustained release, and to study the release characteristics of the drug loaded microspheres in vitro. Methods the compound emulsion solvent volatilization method was used. OPG-PLGA microspheres were prepared with different stirring speed and PVA concentration and their drug loading and encapsulation efficiency were determined. The preparation conditions were optimized by orthogonal test. The release characteristics of drug loaded microspheres were investigated by using phosphate buffer solution as release medium. Results the drug loaded microspheres prepared with the concentration of PLGA polymer 400 mg / ml and stirring speed of 400 r / min PVA concentration 2% had the best drug loading and encapsulation efficiency. The drug release test in vitro showed that the sustained release time of the microspheres was 30 days, and the sustained release effect was good. Conclusion the OPG-PLGA microspheres prepared under the optimized conditions have higher encapsulation efficiency, higher drug loading and better sustained release effect. It provides a basis for the study of sustained-release drugs for tooth extraction site preservation.
【作者单位】：郑州大学第一附属医院口腔种植科;四川大学华西口腔医院粘膜科;
【基金】：河南省医学科技攻关计划项目(编号:201303096)
【分类号】：R782.1

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