预培养比格犬干细胞的牙本质片复合牙周膜细胞膜片裸鼠体内移植研究

发布时间：2018-03-11 16:26

本文选题：牙周膜细胞　切入点：骨髓基质细胞　出处：《实用口腔医学杂志》2014年05期 　论文类型：期刊论文

【摘要】：目的:探索预培养干细胞的牙本质片复合牙周膜细胞(PDLCs)膜片对牙周组织再生的可行性。方法:将比格犬牙根制成牙本质片,其上接种骨髓基质干细胞(BMSCs)或PDLCs,应用成骨诱导液或α-MEM培养液培养,电镜观察牙本质片表面细胞附着与基质形成情况。并构建细胞/牙片/膜片/煅烧陶瓷牛骨CBB复合体,裸鼠体内皮下移植,8周后取材HE染色观察。结果:预培养干细胞的牙本质片,电镜检测到表面有足够的细胞和细胞外基质分布。裸鼠体内结果显示牙本质片接种BMSCs或PDLCs经成骨诱导组可见类牙骨质基质形成,但无类牙周膜纤维,未经成骨诱导组无类牙骨质基质形成,但有明显的类牙周膜纤维样组织形成;空白组无类牙骨质基质及类牙周膜纤维样组织形成。结论:在牙本质片上预培养BMSCs或PDLCs,复合PDLC膜片和CBB后异位移植,有利于类牙周膜纤维的形成。加入成骨诱导液诱导,有利于基质的形成。
[Abstract]:Objective: to explore the feasibility of periodontal tissue regeneration by PDLCs membrane of precultured stem cells. Bone marrow mesenchymal stem cells (BMSCs) or PDLCswere inoculated on it and cultured in osteogenic medium or 伪 -MEM culture medium. The adhesion and matrix formation on the surface of dentin were observed by electron microscope. The CBB complex of cell / dental slice / membrane / calcined ceramic bovine bone was constructed. After 8 weeks of subcutaneous transplantation in nude mice, HE staining was used. Results: dental slices of pre-cultured stem cells, Enough cells and extracellular matrix were found on the surface of nude mice by electron microscope. In vivo, dentin tablets inoculated with BMSCs or PDLCs could be seen to form cementoid matrix, but no periodontal ligament fibers were found in osteogenic induction group. No cementoid matrix was formed in the group without osteogenesis, but the fibrous tissue of periodontal ligament was obviously formed. Conclusion: preculture of BMSCs or PDLCs on dentin plate, combination of PDLC membrane and CBB were beneficial to the formation of periodontal ligament fiber, and the addition of osteoblastic inducer was beneficial to the formation of periodontal ligament fiber. It is favorable to the formation of matrix.
【作者单位】：上海口腔生物医学及转化医学实验室同济大学口腔医学院牙周科;
【基金】：国家自然科学基金(编号:81271152)
【分类号】：R781

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