TCDD诱导胎鼠腭裂的实验研究进展

发布时间：2018-03-16 21:40

本文选题：-四氯二苯二英　切入点：胎鼠　出处：《重庆医科大学学报》2017年12期 　论文类型：期刊论文

【摘要】：目的:总结归纳2,3,7,8-四氯二苯二英(2,3,7,8-tetrachlorodibenzo-p-dioxin,TCDD)诱导胎鼠腭裂的实验研究现状,为深入探讨TCDD致胎鼠腭裂的发生机制提供新的思路。方法:通过中国知网、NCBI等国内外文献库检索"TCDD""腭裂"等关键词,搜集已有文献资料。结果:目前的资料显示,可以通过约20μg/kg剂量的TCDD给予孕第9~14天的小鼠单次灌胃建立稳定的胎鼠腭裂模型。在此基础上发现,TCDD诱导不涉及腭突体积变化,主要通过抑制腭突接触融合而发挥致腭裂效应。对于发育中的腭组织细胞,TCDD能改变一系列调节细胞增殖、分化、凋亡的生长因子及其受体的表达,主要包括表皮细胞生长因子(epidermal growth factor,EGF)、转化生长因子(transforming growth factor,TGF)、芳香烃受体(aryl hydrocarbon receptor,AhR)。这些分子的表达变化尚存在争议。腭融合过程中发挥关键作用的腭中缝上皮细胞(medial edge epithelium,MEE),其细胞命运转归尚未阐明。结论:TCDD诱导胎鼠腭裂的体内实验基础相对完善。腭发育调控机制纷繁复杂,注重器官发育的时空动态性对于进一步研究至关重要。腭器官离体培养将是重要的体外实验环节。实验技术和条件的变革对于本领域的研究进展显得愈加迫切。
[Abstract]:Objective: to summarize the present situation of the experimental study on the cleft palate induced by TCDD in the fetal rats. In order to provide a new idea for exploring the mechanism of cleft palate induced by TCDD, methods: the key words of "TCDD" and "cleft palate" were searched by the domestic and foreign literature database of TCDD. Results: the present data showed that the cleft palate might be caused by cleft palate. A stable cleft palate model of fetal mice could be established by a single dose of 20 渭 g / kg TCDD on the 14th day of gestation, and it was found that the volume of palatine process was not involved in the induction of TCDD. TCDD can change the expression of a series of growth factors and its receptors that regulate cell proliferation, differentiation and apoptosis. It mainly includes epidermal growth factor (EGFN), transforming growth factor (TGFN) and aryl hydrocarbon receptor (AhRN). The expression of these molecules is still in dispute. The medial edge epithelium MEE, which plays a key role in palatal fusion, plays a key role in the process of palatal fusion. Conclusion the in vivo experimental basis of fetal cleft palate induced by TCDD is relatively perfect, and the mechanism of palatal development regulation is complicated. The emphasis on spatiotemporal dynamics of organ development is very important for further research. In vitro culture of palatal organs will be an important experimental link in vitro. The changes of experimental techniques and conditions are becoming more and more urgent for the research progress in this field.
【作者单位】：重庆医科大学附属儿童医院烧伤整形科儿童发育疾病研究教育部重点实验室重庆市儿科学重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地;
【基金】：国家自然科学基金青年基金资助项目(编号:81202167) 重庆市渝中区科技计划资助项目(编号:20130121、20150112) 国家临床重点专科建设资助项目(编号:国卫办医函[2013]544)
【分类号】：R782.22

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