牙龈卟啉单胞菌对兔血管内皮组织中白细胞介素-33表达的影响

发布时间：2018-03-19 23:31

本文选题：牙龈卟啉单胞菌　切入点：血管内皮组织　出处：《华西口腔医学杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的用牙龈卟啉单胞菌感染兔建立动脉粥样硬化(AS)模型,观察感染兔主动脉血管内皮细胞中白细胞介素-33(IL-33)的变化,探讨牙龈卟啉单胞菌与AS的关系。方法将24只新西兰大白兔分为对照组和实验组。实验组每周1次静脉注射牙龈卟啉单胞菌培养液,持续12周,建立感染兔AS模型;对照组每周1次注射等量生理盐水。实验13周处死实验动物,观察主动脉血管的组织结构;通过免疫组织化学染色、实时荧光半定量聚合酶链式反应和Western blot法检测兔主动脉血管内皮细胞中IL-33 m RNA和蛋白质的表达。结果实验组血管内皮细胞IL-33 m RNA相对表达量为58.244±2.407,IL-33蛋白相对表达量为1.863±0.171,对照组IL-33 m RNA相对表达量为3.143±0.805,IL-33蛋白相对表达量为0.537±0.028;实验组IL-33的m RNA和蛋白的表达量均明显高于对照组(P0.01)。结论牙龈卟啉单胞菌感染能促进血管内皮细胞表达IL-33,可能对AS的发生发展有一定的调节作用。
[Abstract]:Objective to establish the atherosclerotic atherosclerosis model in rabbits infected with porphyromonas gingivalis and to observe the changes of interleukin-33 (IL-33) in vascular endothelial cells (VEC) of aorta of rabbits infected with porphyromonas gingivalis. Methods 24 New Zealand rabbits were divided into two groups: control group and experimental group. The control group was injected with the same amount of saline once a week. After 13 weeks of experiment, the experimental animals were killed to observe the tissue structure of aortic vessels. Real-time fluorescence semi-quantitative polymerase chain reaction and Western blot assay were used to detect the expression of IL-33 m RNA and protein in rabbit aortic endothelial cells. Results the relative expression of IL-33 m RNA in experimental group was 58.244 卤2.407 and IL-33 protein expression was 58.244 卤2.407. The relative expression of IL-33 m RNA was 3. 143 卤0. 805 and 0. 537 卤0. 028 in the control group, and the expression of m RNA and protein in IL-33 in the experimental group was significantly higher than that in the control group P0. 01. Conclusion Porphyromonas gingivalis infection can promote the expression of IL-33 in vascular endothelial cells. It may play a role in regulating the occurrence and development of as.
【作者单位】：佳木斯大学附属口腔医院牙周黏膜病科;
【基金】：黑龙江省教育厅科研基金(12531707) 佳木斯大学研究生科研创新基金(LM2014-041)~~
【分类号】：R781

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