多种诱发小鼠舌癌模型方法的比较

发布时间：2018-03-20 01:10

本文选题：小鼠　切入点：-硝基喹啉--氧化物　出处：《暨南大学学报(自然科学与医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的:建立小鼠舌癌模型,筛选诱癌率高及模拟人类发病的舌癌模型.方法:以Babl/c小鼠为实验对象,通过单独和联合应用4-硝基喹啉-1-氧化物(4NQO)和槟榔碱(Arecoline)来诱发舌癌,以涂布法采用质量浓度5mg/mL 4NQO;自然饮水法采用质量浓度20、50、100μg/mL 4NQO,采用质量浓度300μg/mL Arecoline及联合质量浓度100μg/mL 4NQO和质量浓度300μg/mL Arecoline.各组诱癌剂饮用至16或14周时,改为饮用灭菌自来水观察至第40周,于诱癌的不同节点选取状态不佳,体质量下降超过20%的小鼠处死,取其舌头、食管、胃、肝、脾脏组织标本,通过肉眼和组织学观察肿瘤发生发展情况.结果:自然饮水法质量浓度50μg/mL 4NQO组70.0%(7/10)为轻度不典型增生,40.0%(4/10)为中度不典型增生,20.0%(2/10)为重度不典型增生,10.0%(1/10)为原位癌;联合应用质量浓度为100μg/mL 4NQO和质量浓度为300μg/mL Arecoline组10.0%(1/10)为轻度不典型增生,20.0%(2/10)为中度不典型增生,40.0%(4/10)为重度不典型增生,20.0%(2/10)为原位癌,50.0%(5/10)为浸润癌,30.0%(3/10)淋巴结转移,20.0%(2/10)食管不典型增生.结论:质量浓度50μg/mL的4NQO饮用16周继续观察至40周是舌癌癌前病变动物模型建立的理想时间;质量浓度为100μg/mL的4NQO饮用14周后改饮用自来水至40周癌变发展缓慢、典型,发病率高,组织病理学特征与人相似.联合运用低剂量Arecoline和4NQO是诱癌率最高的建模方法,且可观察到颈部淋巴结转移及食管病变,但癌变进展快,不宜作为模拟人舌癌发病的模型.
[Abstract]:Objective: to establish a model of tongue cancer in mice, and to screen a model of tongue cancer with high carcinogenesis rate and mimic human disease. Methods: Babl/c mice were used to induce tongue cancer by using 4NQO (4-nitroquinoline-1-oxide) and Arecoline alone and in combination with arecoline. 5 mg / mL 4NQO was used for coating method, 20 渭 g / mL 4NQO for natural drinking water, 300 渭 g / mL Arecoline and combined concentration of 100 渭 g / mL 4NQO and 300 渭 g / mL Arecoline for 16 or 14 weeks. After drinking sterilized tap water for 40 weeks, the mice whose body mass decreased by more than 20% were killed at the different nodes of cancer induction, and their tongue, esophagus, stomach, liver and spleen were taken out, and the tissues of tongue, esophagus, stomach, liver and spleen were collected. Results: the mass concentration of 50 渭 g / mL 4NQO group (70.010 / 10) was mild atypical hyperplasia (40.010%) and moderate atypical hyperplasia (20.0% / 10) (severe dysplasia 10.0 / 110). Combined use of 100 渭 g / mL 4NQO and 300 渭 g / mL Arecoline group (10.0 / 10) as mild atypical hyperplasia 20.0 / 10) as moderate atypical hyperplasia (40.010 / 10) as severe atypical hyperplasia 20.0% / 10) as carcinoma in situ (50.0% / 510) as invasive carcinoma 30.00.10 / 10) lymph node metastasis 20.0r.r.210. Conclusion: it is an ideal time to establish the animal model of precancerous lesions of the tongue after drinking 4NQO at a concentration of 50 渭 g / mL for 16 weeks to 40 weeks. The carcinogenesis of 4NQO with 100 渭 g / mL 4NQO for 14 weeks to 40 weeks after drinking was slow, typical, high incidence, histopathological characteristics were similar to that of human. The combination of low dose Arecoline and 4NQO was the best method to induce cancer. Cervical lymph node metastasis and esophageal lesions can be observed, but the carcinogenesis is rapid, so it is not suitable to be used as a model for simulating human tongue cancer.
【作者单位】：广西医科大学护理学院基础教研室;广西医科大学病理生理学教研室;广西医科大学病理教研室;
【基金】：广西自然科学基金资助项目(2013GXNSFAA019244)
【分类号】：R-332;R739.86

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