Noth2调控舌鳞癌干细胞的增殖、侵袭和迁移

发布时间：2018-03-21 16:05

本文选题：干细胞　切入点：肿瘤干细胞　出处：《中国组织工程研究》2016年45期 　论文类型：期刊论文

【摘要】：背景:前期研究发现,Notch2在舌鳞癌干细胞ALDH~(br)中表达显著上调,但其具体的作用尚不明确。目的:分析Notch2对舌鳞癌干细胞ALDH~(br)增殖、侵袭和迁移的影响。方法:采用无血清培养基培养舌鳞癌细胞株Tca8113,富集ALDH~(br)细胞,以流式细胞技术对其进行分选,采用免疫印迹检测Notch2在普通舌鳞癌肿瘤细胞ALDHlow和ALDH~(br)细胞中的表达水平。通过脂质体转染Notch2 sh RNA(sh-Notch2)构建Notch2沉默的ALDH~(br)细胞(实验组),以转染sh-control的ALDH~(br)细胞为对照,48 h后检测ALDH~(br)细胞的增殖及Ki67蛋白表达;Transwell小室法检测两组细胞生物侵袭、迁移及基质金属蛋白酶2和基质金属蛋白酶9蛋白的表达。结果与结论:(1)Notch2在ALDH~(br)细胞中的表达水平显著高于其在ALDHlow细胞中的表达水平(P0.05);(2)实验组ALDH~(br)细胞的增殖能力、Ki67蛋白表达低于对照组(P0.05);(3)实验组ALDH~(br)细胞的侵袭和迁移能力显著低于对照组(P0.05),基质金属蛋白酶2和基质金属蛋白酶9的表达水平显著低于对照组(P0.05);(4)结果表明,Notch2可促进ALDH~(br)细胞的增殖、侵袭和迁移,提示Notch2在舌鳞癌的发生与发展中起着重要作用,其可能成为治疗舌鳞癌的分子靶点。
[Abstract]:Background: the expression of Notch2 was significantly up-regulated in tongue squamous cell carcinoma stem cells, but its specific role was not clear. Objective: to analyze the effect of Notch2 on the proliferation of tongue squamous cell carcinoma stem cells. Methods: tongue squamous cell line Tca8113 was cultured in serum-free medium to enrich ALDHN br.These cells were separated by flow cytometry. Western blotting was used to detect the expression of Notch2 in normal tongue squamous cell carcinoma cells ALDHlow and ALDHHtch2.The silencing Notch2 silenced ALDHHtch2 cells were constructed by liposome transfection of Notch2 sh RNA-Notch2) cells (experimental group and sh-control transfected ALDHHtch2 cells as control 48 h later). The proliferation and expression of Ki67 protein were measured by transwell chamber assay. Migration and expression of matrix metalloproteinase-2 and matrix metalloproteinase-9. Results and conclusion the expression level of Notch2 in ALDHlow cells was significantly higher than that in ALDHlow cells. The expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 was significantly lower than that of control group (P0.05, P 0.05). The results showed that Notch2 could promote the proliferation of ALDHHbrbrB cells, and the expression of matrix metalloproteinase 2 and matrix metalloproteinase-9 was significantly lower than that of control group (P 0.05). Invasion and migration suggest that Notch2 plays an important role in the occurrence and development of tongue squamous cell carcinoma and may become a molecular target for the treatment of tongue squamous cell carcinoma.
【作者单位】：六盘水市人民医院口腔科;郑州大学第二附属医院康复科;六盘水市人民医院肿瘤科;贵州医科大学天然药物资源优效利用重点实验室;
【分类号】：R739.86

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