实验性牙周炎对肥胖大鼠血清C反应蛋白及胰岛素抵抗的影响

发布时间：2018-03-23 11:39

本文选题：实验性牙周炎　切入点：肥胖　出处：《实用口腔医学杂志》2017年03期

【摘要】：目的:探讨实验性牙周炎对肥胖大鼠血清C反应蛋白(CRP)及胰岛素抵抗指数(HOMA-IR)随时间的变化。方法:4周龄SD大鼠(n=35)高脂饲料喂养16周建立肥胖模型。按肥胖指标筛除5只,其余30只肥胖大鼠按1∶2比例,随机选取10只大鼠为肥胖组,20只肥胖大鼠牙周丝线结扎建立肥胖复合牙周炎模型(复合组),结扎后有4只大鼠死亡。在牙周结扎前、结扎1周、4周3个时间点眼眶静脉取血,检测空腹血糖及空腹胰岛素,并计算HOMA-IR及β细胞功能指数(HOMA-β);ELISA检测血清CRP水平。结果:牙周结扎1周后,复合组CRP水平显著升高,在结扎4周时则逐渐回落(F=7.773,P=0.004)。牙周结扎后4周,复合组HOMA-IR显著高于肥胖组(F=-4.691,P=0.000),HOMA-β显著低于对照组(F=3.672,P=0.002)。结论:实验性牙周炎影响肥胖大鼠血清CRP水平;实验性牙周炎会加重肥胖大鼠的胰岛素抵抗状态并下调β细胞功能。
[Abstract]:Objective: to investigate the changes of serum C-reactive protein (CRP) and insulin resistance index (HOMA-IRR) in obese rats treated with experimental periodontitis. The other 30 obese rats were randomly selected as obese group at 1:2, 20 obese rats were ligated with periodontal filaments to establish the model of obesity combined periodontitis (compound group, 4 rats died after ligation, before periodontal ligation, 4 rats died before periodontal ligation, 4 rats died after ligation, 4 rats died after ligation, 4 rats died after ligation, 4 rats died after ligation, 4 rats died after ligation. Blood samples were collected from orbital vein at 3 points in 1 week and 4 weeks after ligation, fasting blood glucose and fasting insulin were measured, and serum CRP levels were measured by HOMA-IR and 尾 -cell function index. Results: after 1 week of periodontal ligation, the CRP level in compound group was significantly higher than that in control group. After 4 weeks of ligation, the HOMA-IR of the compound group was significantly higher than that of the obese group. The level of serum CRP was significantly lower in the compound group than that in the control group. Conclusion: experimental periodontitis affects the serum CRP level of obese rats. Experimental periodontitis exacerbates insulin resistance and down-regulates 尾-cell function in obese rats.
【作者单位】：南方医科大学口腔医院·广东省口腔医院;中国科学院大学存济医学院;
【基金】：国家自然科学基金(编号:81300890)
【分类号】：R781.4

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