新型低聚合收缩树脂材料的体外细胞毒性研究

发布时间：2018-03-23 12:16

本文选题：新型3F-PAc树脂基质　切入点：Bis-GMA　出处：《吉林大学》2017年硕士论文

【摘要】：简介:复合树脂类牙科修复用材料因其操作简单快捷,色泽美观性佳,与天然牙相似度高而越来越被普遍应用于口腔科的修复治疗中,然而尽管随着材料工艺的不断发展,复合树脂类材料有了很大的进步,但仍旧存在着三大缺陷,即聚合收缩、耐磨性差及生物相容性的问题。近年来,随着生活水平的提高,口腔材料的生物安全性问题逐渐引起人们的重视,而树脂材料的聚合收缩率与材料的生物安全性具有一定的相关性,基于以上原因推动了国内外学者致力于研发出新型的机械性能更好,安全性更佳的口腔修复用牙科树脂材料。每一种新研发的口腔材料更需经过生物安全性检测,细胞毒性检测是其中一项重要的参考内容,本研究即针对一种新研发的具有较低聚合收缩性能的树脂材料的细胞毒性性进行检测,为该复合树脂材料在临床应用提供生物安全性的依据,同时也对树脂材料中的一种重要稀释剂TEGDMA成分对树脂材料细胞毒性的影响做了初步研究。目的:评价一种新型低聚合收缩树脂材料的浸提液对L929小鼠成纤维细胞的细胞毒性,以及不同浓度梯度的材料浸提液对细胞增殖的影响,进而判断其毒性的大小,并初步分析复合树脂基质中的稀释剂TEGDMA对材料毒性的影响,为后续研究奠定基础。方法:由本课题组于实验室合成新型低聚合收缩树脂基质——间三氟甲基苯基型丙烯酸酯3F-PAc,与临床上传统常用树脂基质Bis-GMA作比较,将实验组分为三组,分别为Bis-GMA组、3F-PAc 1组和3F-PAc 0组,3F-PAc 0组不添加稀释剂TEGDMA。制备三组材料浸提液,连续稀释为4种浓度100%、50%、25%、12.5%;采用甲基噻唑基四唑比色法(methyl thiazolyl tetrazolium,MTT)检测小鼠成纤维细胞L929与各稀释液共培养1、3、5d后的细胞相对增值率并与阴性对照组作比较,评价细胞毒性等级。阴性对照组只加含10%胎牛血清的DMEM完全培养基,阳性对照组为0.64%苯酚的浸提液。结果与结论:三组材料的浸渍液均表现出了程度不等的细胞毒性作用,除培养3d和5d时Bis-GMA和3F-PAc 1组的100%浸液毒性等级为2级外,其余各实验组浸提液细胞毒性的等级在0~1级之间。3F-PAc 0组浸提液吸光度值大于3F-PAc1组与Bis-GMA组,其差异具有统计学意义(P0.05),3F-PAc 1组与Bis-GMA组之间差异不显著(P0.05)。综上所述得出结论:三种复合树脂材料的细胞毒性的评级都在安全的范围内,新型3F-PAc树脂基质材料细胞毒性等级符合标准,具有较好的生物安全性;稀释剂TEGDMA可能对树脂材料的细胞毒性有一定影响。
[Abstract]:Summary: composite resin dental restoration materials are more and more widely used in stomatology because of their simple and quick operation, beautiful color and high similarity with natural teeth. However, with the development of material technology, Composite resin materials have made great progress, but there are still three major defects, namely, polymerization shrinkage, poor wear resistance and biocompatibility. In recent years, with the improvement of living standards, People pay more and more attention to the biosafety of oral materials, and the polymerization shrinkage rate of resin materials has a certain correlation with the biosafety of materials. For these reasons, scholars at home and abroad are committed to developing new dental resin materials with better mechanical properties and better safety. Cytotoxicity detection is one of the important reference contents. In this study, the cytotoxicity of a new resin material with low polymerization shrinkage was studied. It provides the basis of biosafety for the clinical application of the composite resin material. At the same time, the effects of an important diluent TEGDMA on the cytotoxicity of resin materials were studied. Objective: to evaluate the cytotoxicity of a new type of low polymerization shrinkage resin to L929 mouse fibroblasts. The effects of different concentration gradient material extract on cell proliferation and the toxicity of TEGDMA in composite resin matrix were also analyzed, and the effects of the diluent TEGDMA in the composite resin matrix on the toxicity of the composite resin matrix were preliminarily analyzed, and the effects of the diluent TEGDMA on the toxicity of the composite resin matrix were analyzed. Methods: a new type of low polymerization shrinkage resin matrix, m-trifluoromethylphenyl acrylate (3F-PAc), was synthesized in our laboratory. The experimental group was divided into three groups, compared with the traditional resin matrix Bis-GMA. Three groups of material extracts were prepared from Bis-GMA group and 3F-PAc group 0 group without adding diluent, respectively. The relative proliferation rate of mouse fibroblasts L929 was detected by methyl thiazolyl tetrazolium MTT, and the cell proliferation rate was compared with that of the negative control group after co-cultured with each diluent for 5 days. To evaluate the cytotoxicity grade, the negative control group was only added with 10% fetal bovine serum DMEM complete medium, and the positive control group was 0.64% phenol extract. Results and conclusion: the three groups showed different cytotoxic effects. Except that the toxicity grade of 100% extract of Bis-GMA and 3F-PAc 1 group was grade 2 at 3 and 5 days after culture, the cell toxicity of the other experimental groups was higher than that of 3F-PAc1 group and Bis-GMA group in the range of 0 ~ (-1). 3 F-PAc _ 0 group was higher than that of 3F-PAc1 group and Bis-GMA group. The difference was statistically significant. There was no significant difference between Bis-GMA group and P0.05 group. In summary, it was concluded that the cytotoxicity ratings of the three composite resin materials were all within the safe range. The cytotoxic grade of the new 3F-PAc resin matrix material is up to the standard and has good biological safety, and the diluent TEGDMA may have some influence on the cytotoxicity of the resin material.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.1

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